Li Zhang1, Limin Zhang2, Haiqin Zhang3, Wenjun Wang4, You Zhao5. 1. Department of Pain, Yidu Central Hospital of Weifang, Qingzhou, Shangdong, China. 2. Department of Gynecology, Maternity and Child Health Hospital of Weifang, Weifang, Shangdong, China. 3. Department of Anesthesiology, Yidu Central Hospital of Weifang, Qingzhou, Shangdong, China. 4. The Second Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China. 5. Department of Orthopaedics, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.
Abstract
BACKGROUND: Many studies have revealed that transforming growth factor-beta (TGF-β) signals play important roles in maintaining normal status of articular cartilage in human osteoarthritis (OA). However, SMAD3 had inhibitory effect on TGF-β-induced chondrocyte maturation. METHOD: To evaluate the association of SMAD3 genetic variants with the risk of knee OA, we conducted this hospital-based case-control study involving 350 knee patients with OA and 400 controls in a Chinese population. Genotyping was performed using a custom-by-design 48-Plex single-nucleotide polymorphism (SNP) Scan™ Kit. RESULTS: Our results indicate that the GG genotype of rs12901499 could decrease the risk of knee OA compared to AA genotype. However, stratified analyses by sex and age did not obtain positive findings with regard to the association between rs12901499 polymorphism and knee OA risk. CONCLUSION: In conclusion, SMAD3 rs12901499 polymorphism may be involved in the development of knee OA. Larger studies with more diverse ethnic populations are needed to confirm these results.
BACKGROUND: Many studies have revealed that transforming growth factor-beta (TGF-β) signals play important roles in maintaining normal status of articular cartilage in human osteoarthritis (OA). However, SMAD3 had inhibitory effect on TGF-β-induced chondrocyte maturation. METHOD: To evaluate the association of SMAD3 genetic variants with the risk of knee OA, we conducted this hospital-based case-control study involving 350 knee patients with OA and 400 controls in a Chinese population. Genotyping was performed using a custom-by-design 48-Plex single-nucleotide polymorphism (SNP) Scan™ Kit. RESULTS: Our results indicate that the GG genotype of rs12901499 could decrease the risk of knee OA compared to AA genotype. However, stratified analyses by sex and age did not obtain positive findings with regard to the association between rs12901499 polymorphism and knee OA risk. CONCLUSION: In conclusion, SMAD3rs12901499 polymorphism may be involved in the development of knee OA. Larger studies with more diverse ethnic populations are needed to confirm these results.
Authors: Tian-Fang Li; Michael Darowish; Michael J Zuscik; Di Chen; Edward M Schwarz; Randy N Rosier; Hicham Drissi; Regis J O'Keefe Journal: J Bone Miner Res Date: 2005-09-19 Impact factor: 6.741