Literature DB >> 29292806

Structural basis for controlling the enzymatic properties of polymannuronate preferred alginate lyase FlAlyA from the PL-7 family.

Hui-Min Qin1, Takuya Miyakawa, Akira Inoue, Ryuji Nishiyama, Akira Nakamura, Atsuko Asano, Takao Ojima, Masaru Tanokura.   

Abstract

FlAlyA is an endolytic enzyme with a preference for polymannuronate. The crystal structure and mutagenesis studies elucidated that the structural variations at outer uronate-binding subsites +2, +3 and -2 control the enzymatic properties of PL-7 family enzymes. Lys158 mutations changed the pH dependency and enhanced the production of mono- and disaccharides.

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Year:  2018        PMID： 29292806     DOI： 10.1039/c7cc06523j

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  10 in total

1.  Structural and molecular basis for the substrate positioning mechanism of a new PL7 subfamily alginate lyase from the arctic.

Authors:  Fei Xu; Xiu-Lan Chen; Xiao-Hui Sun; Fang Dong; Chun-Yang Li; Ping-Yi Li; Haitao Ding; Yin Chen; Yu-Zhong Zhang; Peng Wang
Journal:  J Biol Chem       Date:  2020-09-23       Impact factor: 5.157

2.  Characterization of a Novel PolyM-Preferred Alginate Lyase from Marine Vibrio splendidus OU02.

Authors:  Jingjing Zhuang; Keke Zhang; Xiaohua Liu; Weizhi Liu; Qianqian Lyu; Aiguo Ji
Journal:  Mar Drugs       Date:  2018-08-22       Impact factor: 5.118

3.  Genome-Wide Analysis of PL7 Alginate Lyases in the Genus Zobellia.

Authors:  Nadezhda Chernysheva; Evgeniya Bystritskaya; Galina Likhatskaya; Olga Nedashkovskaya; Marina Isaeva
Journal:  Molecules       Date:  2021-04-20       Impact factor: 4.411

Review 4.  Structure Characteristics, Biochemical Properties, and Pharmaceutical Applications of Alginate Lyases.

Authors:  Shu-Kun Gao; Rui Yin; Xiao-Chen Wang; Hui-Ning Jiang; Xiao-Xiao Liu; Wei Lv; Yu Ma; Yan-Xia Zhou
Journal:  Mar Drugs       Date:  2021-11-10       Impact factor: 5.118

5.  Biochemical characterization and biocatalytic application of a novel d-tagatose 3-epimerase from Sinorhizobium sp.

Authors:  Zhangliang Zhu; Chao Li; Xin Liu; Dengke Gao; Xueyu Wang; Masaru Tanokura; Hui-Min Qin; Fuping Lu
Journal:  RSC Adv       Date:  2019-01-22       Impact factor: 4.036

6.  Determination of oligosaccharide product distributions of PL7 alginate lyases by their structural elements.

Authors:  Keke Zhang; Zhijian Li; Qiaoyun Zhu; Huansheng Cao; Xinxin He; Xiao-Hua Zhang; Weizhi Liu; Qianqian Lyu
Journal:  Commun Biol       Date:  2022-08-02

7.  Biochemical Characterization and Elucidation of the Hybrid Action Mode of a New Psychrophilic and Cold-Tolerant Alginate Lyase for Efficient Preparation of Alginate Oligosaccharides.

Authors:  Shengsheng Cao; Li Li; Benwei Zhu; Zhong Yao
Journal:  Mar Drugs       Date:  2022-08-05       Impact factor: 6.085

8.  Cloning, Secretory Expression and Characterization of a Unique pH-Stable and Cold-Adapted Alginate Lyase.

Authors:  Zhi-Peng Wang; Min Cao; Bing Li; Xiao-Feng Ji; Xin-Yue Zhang; Yue-Qi Zhang; Hai-Ying Wang
Journal:  Mar Drugs       Date:  2020-04-01       Impact factor: 5.118

9.  Engineering of 3-ketosteroid-∆1-dehydrogenase based site-directed saturation mutagenesis for efficient biotransformation of steroidal substrates.

Authors:  Shuhong Mao; Jian-Wen Wang; Fufeng Liu; Zhangliang Zhu; Dengke Gao; Qianqian Guo; Panpan Xu; Zheng Ma; Yali Hou; Xiaotao Cheng; Dengyue Sun; Fuping Lu; Hui-Min Qin
Journal:  Microb Cell Fact       Date:  2018-09-10       Impact factor: 5.328

Review 10.  Bacterial alginate metabolism: an important pathway for bioconversion of brown algae.

Authors:  Lanzeng Zhang; Xue Li; Xiyue Zhang; Yingjie Li; Lushan Wang
Journal:  Biotechnol Biofuels       Date:  2021-07-18       Impact factor: 6.040
  10 in total

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