Literature DB >> 2928334

Polycythemia in transgenic mice expressing the human erythropoietin gene.

G L Semenza1, M D Traystman, J D Gearhart, S E Antonarakis.   

Abstract

Erythropoietin is a glycoprotein hormone that regulates mammalian erythropoiesis. To study the expression of the human erythropoietin gene, EPO, 4 kilobases of DNA encompassing the gene with 0.4 kilobase of 5' flanking sequence and 0.7 kilobase of 3' flanking sequence was microinjected into fertilized mouse eggs. Transgenic mice were generated that are polycythemic, with increased erythrocytic indices in peripheral blood, increased numbers of erythroid precursors in hematopoietic tissue, and increased serum erythropoietin levels. Transgenic homozygotes show a greater degree of polycythemia than do heterozygotes as well as striking extramedullary erythropoiesis. Human erythropoietin RNA was found not only in fetal liver, adult liver, and kidney but also in all other transgenic tissues analyzed. Anemia induced increased human erythropoietin RNA levels in liver but not kidney. These transgenic mice represent a unique model of polycythemia due to increased erythropoietin levels.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2928334      PMCID： PMC286900          DOI： 10.1073/pnas.86.7.2301

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  24 in total

1.  Cloning, sequencing, and evolutionary analysis of the mouse erythropoietin gene.

Authors:  J D McDonald; F K Lin; E Goldwasser
Journal:  Mol Cell Biol       Date:  1986-03       Impact factor: 4.272

2.  Cloning and expression of the human erythropoietin gene.

Authors:  F K Lin; S Suggs; C H Lin; J K Browne; R Smalling; J C Egrie; K K Chen; G M Fox; F Martin; Z Stabinsky
Journal:  Proc Natl Acad Sci U S A       Date:  1985-11       Impact factor: 11.205

Review 3.  Renal erythropoietin: properties and production.

Authors:  W Jelkmann
Journal:  Rev Physiol Biochem Pharmacol       Date:  1986       Impact factor: 5.545

4.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1983-07-01       Impact factor: 3.365

5.  Complementary DNA sequence of a human cytoplasmic actin. Interspecies divergence of 3' non-coding regions.

Authors:  I Hanukoglu; N Tanese; E Fuchs
Journal:  J Mol Biol       Date:  1983-02-05       Impact factor: 5.469

6.  Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs.

Authors:  R L Brinster; H Y Chen; M E Trumbauer; M K Yagle; R D Palmiter
Journal:  Proc Natl Acad Sci U S A       Date:  1985-07       Impact factor: 11.205

7.  Anemia induces accumulation of erythropoietin mRNA in the kidney and liver.

Authors:  M C Bondurant; M J Koury
Journal:  Mol Cell Biol       Date:  1986-07       Impact factor: 4.272

8.  Expression of the erythropoietin gene.

Authors:  N Beru; J McDonald; C Lacombe; E Goldwasser
Journal:  Mol Cell Biol       Date:  1986-07       Impact factor: 4.272

9.  Chromosomal assignment of the human erythropoietin gene and its DNA polymorphism.

Authors:  M L Law; G Y Cai; F K Lin; Q Wei; S Z Huang; J H Hartz; H Morse; C H Lin; C Jones; F T Kao
Journal:  Proc Natl Acad Sci U S A       Date:  1986-09       Impact factor: 11.205

10.  Murine erythropoietin gene: cloning, expression, and human gene homology.

Authors:  C B Shoemaker; L D Mitsock
Journal:  Mol Cell Biol       Date:  1986-03       Impact factor: 4.272
View more
  38 in total

1.  A nuclear factor induced by hypoxia via de novo protein synthesis binds to the human erythropoietin gene enhancer at a site required for transcriptional activation.

Authors:  G L Semenza; G L Wang
Journal:  Mol Cell Biol       Date:  1992-12       Impact factor: 4.272

Review 2.  Animal models for the biological effects of continuous high cytokine levels.

Authors:  M Lübbert; D Jonas; F Herrmann
Journal:  Blut       Date:  1990-11

3.  Profile of Gregg L. Semenza.

Authors:  Farooq Ahmed
Journal:  Proc Natl Acad Sci U S A       Date:  2010-08-02       Impact factor: 11.205

Review 4.  Erythropoietic and non-erythropoietic functions of erythropoietin in mouse models.

Authors:  Johannes Vogel; Max Gassmann
Journal:  J Physiol       Date:  2011-01-31       Impact factor: 5.182

Review 5.  Transgenic mice in the study of cytokine function.

Authors:  J Taverne
Journal:  Int J Exp Pathol       Date:  1993-12       Impact factor: 1.925

6.  Bovine alpha s1-casein gene sequences direct high level expression of human granulocyte-macrophage colony-stimulating factor in the milk of transgenic mice.

Authors:  M Uusi-Oukari; J M Hyttinen; V P Korhonen; A Västi; L Alhonen; O A Jänne; J Jänne
Journal:  Transgenic Res       Date:  1997-01       Impact factor: 2.788

7.  Myoblast transfer of human erythropoietin gene in a mouse model of renal failure.

Authors:  Y Hamamori; B Samal; J Tian; L Kedes
Journal:  J Clin Invest       Date:  1995-04       Impact factor: 14.808

Review 8.  Erythropoietin.

Authors:  H Franklin Bunn
Journal:  Cold Spring Harb Perspect Med       Date:  2013-03-01       Impact factor: 6.915

9.  Isolation of a cosmid clone corresponding to an inv(21) breakpoint of a patient with transient abnormal myelopoiesis.

Authors:  T Ohta; M Nakano; T Tsujita; K Abe; K Osoegawa; T Yamagata; K Yoshiura; Y Jinno; E Soeda; Y Nakamura; N Niikawa
Journal:  Am J Hum Genet       Date:  1996-03       Impact factor: 11.025

10.  Cell-type-specific and hypoxia-inducible expression of the human erythropoietin gene in transgenic mice.

Authors:  G L Semenza; S T Koury; M K Nejfelt; J D Gearhart; S E Antonarakis
Journal:  Proc Natl Acad Sci U S A       Date:  1991-10-01       Impact factor: 11.205
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.