| Literature DB >> 29277714 |
Yu-Ri Park1, Hye-Ryung Kim2, Seong-Hee Kim3, Kyoung-Ki Lee4, Young S Lyoo5, Sang-Geon Yeo6, Choi-Kyu Park7.
Abstract
A loop-mediated isothermal amplification (LAMP) assay using hydroxynaphthol blue was developed for the rapid and visual detection of the capsid gene of porcine circovirus 3 (PCV3). The amplification could be completed in 40 min at 62°C, and the results could be visually detected by the naked eye. The assay specifically amplified PCV3 DNA and not other porcine viral nucleic acids. The limit of detection of the assay was 50 PCV3 DNA copies, which was comparable to that of the real-time polymerase chain reaction (qPCR) and lower than that of conventional PCR. In the clinical evaluation, the PCV3 detection rate of the LAMP assay was higher than that of PCR and agreed 100% with that of qPCR. These results indicate that the LAMP assay will be a valuable tool for the rapid, sensitive, and specific detection of PCV3 in clinical samples.Entities:
Keywords: Capsid gene; Hydroxynaphthol blue; Loop-mediated isothermal amplification; Porcine circovirus 3
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Year: 2017 PMID： 29277714 DOI： 10.1016/j.jviromet.2017.12.006
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014