Cristian Hernández1, Juan Ascacio-Valdés1, Heliodoro De la Garza1, Jorge Wong-Paz2, Cristóbal Noé Aguilar2, Guillermo Cristian Martínez-Ávila3, Cecilia Castro-López3, Antonio Aguilera-Carbó4. 1. Department of Animal Nutrition, Universidad Autónoma Agraria Antonio Narro, Col. Buenavista, Calzada Antonio Narro No. 1923, Saltillo, 25315, Coahuila, Mexico. 2. Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, Blvd. Venustiano Carranza, Col. República, Saltillo, 25280, Coahuila, Mexico. 3. Chemistry and Biochemistry Laboratory, Faculty of Agronomy, Universidad Autónoma de Nuevo León, Francisco I. Madero S/N, Hacienda el Canada, 66050, General Escobedo, NL, Mexico. 4. Department of Animal Nutrition, Universidad Autónoma Agraria Antonio Narro, Col. Buenavista, Calzada Antonio Narro No. 1923, Saltillo, 25315, Coahuila, Mexico. Electronic address: aguilera_carbo@yahoo.com.
Abstract
OBJECTIVE: To determinate the recovery of total polyphenolic compounds content, in vitro antioxidant activity and HPLC/ESI/MS characterization of extract from Nephelium lappaceum L. (Mexican rambutan). METHODS: The rambutan husk extract was obtained by aqueous extraction and a polyphenolic fraction was recovered using Amberlite XAD-16. The total polyphenolic compounds content was determined by the Folin Ciocalteu and butanol-HCI methods. In vitro antioxidant activity was performed using ABTS and ferric reducing antioxidant power methods. RESULTS: Mexican rambutan husk showed a total polyphenolic content of 582 mg/g and an evident antioxidant activity by ABTS and ferric reducing antioxidant power analysis. The HPLC/ESI/MS assay allowed the identification of 13 compounds, most of which belong to ellagitannins. Geraniin, corilagin and ellagic acid were present in the sample; the mineral composition was also evaluated. CONCLUSIONS: Rambutan husk cultivated in Mexico is a promising source for the recovery of added value bioactive compounds with antioxidant activity, which have potential applications as bioactive antioxidant agents for the treatment of diseases.
OBJECTIVE: To determinate the recovery of total polyphenolic compounds content, in vitro antioxidant activity and HPLC/ESI/MS characterization of extract from Nephelium lappaceum L. (Mexican rambutan). METHODS: The rambutanhusk extract was obtained by aqueous extraction and a polyphenolic fraction was recovered using Amberlite XAD-16. The total polyphenolic compounds content was determined by the Folin Ciocalteu and butanol-HCI methods. In vitro antioxidant activity was performed using ABTS and ferric reducing antioxidant power methods. RESULTS: Mexican rambutanhusk showed a total polyphenolic content of 582 mg/g and an evident antioxidant activity by ABTS and ferric reducing antioxidant power analysis. The HPLC/ESI/MS assay allowed the identification of 13 compounds, most of which belong to ellagitannins. Geraniin, corilagin and ellagic acid were present in the sample; the mineral composition was also evaluated. CONCLUSIONS:Rambutanhusk cultivated in Mexico is a promising source for the recovery of added value bioactive compounds with antioxidant activity, which have potential applications as bioactive antioxidant agents for the treatment of diseases.
Authors: Juan Buenrostro-Figueroa; Marcela Mireles; J A Ascacio-Valdés; Antonio Aguilera-Carbo; Leonardo Sepúlveda; Juan Contreras-Esquivel; Raúl Rodríguez-Herrera; C N Aguilar Journal: Iran J Biotechnol Date: 2020-04-01 Impact factor: 1.671
Authors: Luis Estrada-Gil; Juan C Contreras-Esquivel; Carolina Flores-Gallegos; Alejandro Zugasti-Cruz; Mayela Govea-Salas; Marco A Mata-Gómez; Raúl Rodríguez-Herrera; Juan A Ascacio-Valdés Journal: Molecules Date: 2022-02-28 Impact factor: 4.411