Antonio Palumbo1,2, Marco De Martino3, Francesco Esposito3, Filippo Fraggetta4, Pedro N Neto1, Priscila Valverde Fernandes5, Izabella C Santos6, Fernando L Dias6, Luiz E Nasciutti2, Nathalia Meireles Da Costa1, Alfredo Fusco1,3, Luís Felipe Ribeiro Pinto1. 1. Programa de Carcinogênese Molecular, Instituto Nacional de Câncer - INCA, Rio de Janeiro, Brazil. 2. Laboratório de Interações Celulares, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. 3. Istituto di Endocrinologia ed Oncologia Sperimentale del CNR c/o Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Scuola di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli 'Federico II', Naples, Italy. 4. Department of Pathology, Ospedale Cannizzaro, Catania, Italy. 5. Divisão de Patologia, Instituto Nacional de Câncer - INCA, Rua Cordeiro da Graça, Rio de Janeiro, Brazil. 6. Seção de Cirurgia de Cabeça e Pescoço, Instituto Nacional de Câncer - INCA, Praça da Cruz Vermelha, Rio de Janeiro, RJ, Brazil.
Abstract
AIMS: Malignant tumours from the upper aerodigestive tract are grouped collectively in the class of head and neck squamous cell carcinoma (HNSCC). The head and neck tumours were responsible for more than 500 000 cancer cases in 2012, accounting for the sixth highest incidence rate and mortality worldwide among all tumour types. Laryngeal squamous cell carcinoma (LSCC) possesses the second highest incidence rate among all HNSCC. Despite significant advances in surgery and radiotherapy during the last few decades, no treatment has been shown to achieve a satisfactory therapeutic outcome and the mortality rate of LSCC is still high, with a 5-year survival rate of 64%. Therefore, further investigations are required to identify the pathogenesis of LSCC. METHODS AND RESULTS: In order to search for new LSCC biomarkers, we have analysed the expression of the HMGA family members, HMGA1 and HMGA2, by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and immunohistochemistry. HMGA proteins are usually absent in the healthy adult tissues. In contrast, their constitutive expression is a feature of several neoplasias, being associated with a highly malignant phenotype and reduced survival. Here, we report HMGA2 overexpression in larynx carcinomas. Conversely, HMGA1 does not show any differences in its expression between normal and carcinoma tissues. Interestingly, HMGA2 overexpression appears associated with that of two HMGA1-pseudogenes, HMGA1P6 and HMGA1P7, acting as a sponge for HMGA1- and HMGA2-targeting microRNAs and involved in several human cancers. CONCLUSIONS: Therefore, HMGA2 overexpression appears to be a strong feature of larynx carcinoma, supporting its detection as a valid tool for the diagnosis of these malignancies.
AIMS: Malignant tumours from the upper aerodigestive tract are grouped collectively in the class of head and neck squamous cell carcinoma (HNSCC). The head and neck tumours were responsible for more than 500 000 cancer cases in 2012, accounting for the sixth highest incidence rate and mortality worldwide among all tumour types. Laryngeal squamous cell carcinoma (LSCC) possesses the second highest incidence rate among all HNSCC. Despite significant advances in surgery and radiotherapy during the last few decades, no treatment has been shown to achieve a satisfactory therapeutic outcome and the mortality rate of LSCC is still high, with a 5-year survival rate of 64%. Therefore, further investigations are required to identify the pathogenesis of LSCC. METHODS AND RESULTS: In order to search for new LSCC biomarkers, we have analysed the expression of the HMGA family members, HMGA1 and HMGA2, by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and immunohistochemistry. HMGA proteins are usually absent in the healthy adult tissues. In contrast, their constitutive expression is a feature of several neoplasias, being associated with a highly malignant phenotype and reduced survival. Here, we report HMGA2 overexpression in larynx carcinomas. Conversely, HMGA1 does not show any differences in its expression between normal and carcinoma tissues. Interestingly, HMGA2 overexpression appears associated with that of two HMGA1-pseudogenes, HMGA1P6 and HMGA1P7, acting as a sponge for HMGA1- and HMGA2-targeting microRNAs and involved in several humancancers. CONCLUSIONS: Therefore, HMGA2 overexpression appears to be a strong feature of larynx carcinoma, supporting its detection as a valid tool for the diagnosis of these malignancies.
Authors: Marco De Martino; Simona Pellecchia; Francesco Esposito; Nadia Tosti; Cristina Quintavalle; Serenella Eppenberger-Castori; Vincenza Carafa; Alberto Righi; Paolo Chieffi; Alfredo Fusco; Luigi Maria Terracciano; Pierlorenzo Pallante Journal: Cell Cycle Date: 2022-03-14 Impact factor: 5.173
Authors: Marco De Martino; Pedro Nicolau-Neto; Luis Felipe Ribeiro Pinto; Alexandra Traverse-Glehen; Emmanuel Bachy; Vincenzo Gigantino; Rossella De Cecio; Francesco Bertoni; Paolo Chieffi; Alfredo Fusco; Francesco Esposito Journal: Am J Cancer Res Date: 2021-05-15 Impact factor: 6.166
Authors: Marco De Martino; Giuseppe Palma; Claudio Arra; Paolo Chieffi; Alfredo Fusco; Francesco Esposito Journal: Cell Cycle Date: 2020-08-13 Impact factor: 4.534
Authors: Kun Li; Chengcheng Zhang; Lei Chen; Pingping Wang; Yang Fang; Junwei Zhu; Shuo Chen; Juan Du; Bing Shen; Kaile Wu; Yehai Liu Journal: PeerJ Date: 2019-06-11 Impact factor: 2.984
Authors: Nathalia Meireles Da Costa; Luis Felipe Ribeiro Pinto; Luiz Eurico Nasciutti; Antonio Palumbo Journal: Biomed Res Int Date: 2019-10-13 Impact factor: 3.411