| Literature DB >> 29256602 |
Yuan Wu1,2, Liqin Zhang1,2, Cheng Cui2, Sena Cansiz2, Hao Liang1, Cuichen Wu1,2, I-Ting Teng2, Weijun Chen1,3,2, Yuan Liu1,2, Weijia Hou2, Xiaobing Zhang1, Weihong Tan1,3,2.
Abstract
Enhanced targeted gene transduction by AAV2 vectors is achieved by linking the vector to multiple sgc8 aptamers, which are selective for cell membrane protein PTK7. Aptamer molecules are conjugated to multiple sites on a DNA dendrimer (G-sgc8), which is then linked to AAV2 via a dithiobis(succinimidyl propionate) cross-linker containing a disulfide group, which can facilitate the release of AAV2 vectors by reaction with the reduced form of intracellular glutathione. The G-sgc8-AAV2 vectors showed a 21-fold enhancement in binding affinity and an enhanced ability to protect sgc8 aptamers against nuclease degradation to cells expressing PTK7 compared to single aptamer-AAV2 conjugates. The transduction efficiency was tested by loading AAV2 with the gene for green fluorescent protein. Therefore, this modified recombinant vector is an attractive and promising tool for targeted biomedical applications.Entities:
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Year: 2017 PMID： 29256602 PMCID： PMC5877795 DOI： 10.1021/jacs.7b08518
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419