Ha-Rim Kim1, Guem-San Lee2, Mi-Seong Kim1, Do-Gon Ryu3, Hong-Seob So1, Hyoung-Chul Moon4, Young-Rae Lee1,5, Sei-Hoon Yang6, Kang-Beom Kwon7,8. 1. Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan, Jeonbuk, 54538, South Korea. 2. Department of Herbology, Wonkwang University School of Korean Medicine, Iksan, Jeonbuk, 54538, South Korea. 3. Department of Korean Physiology, Wonkwang University School of Korean Medicine, Iksan Jeonbuk, 54538, South Korea. 4. Institute of Customized Physical Therapy, Gwanju, 62279, South Korea. 5. Department of Oral Biochemistry, Wonkwang University School of Dentistry, Iksan, Jeonbuk, 54538, South Korea. 6. Department of Internal Medicine, Wonkwang University School of Medicine, Iksan, Jeonbuk, 54538, South Korea. 7. Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan, Jeonbuk, 54538, South Korea. desson@wku.ac.kr. 8. Department of Korean Physiology, Wonkwang University School of Korean Medicine, Iksan Jeonbuk, 54538, South Korea. desson@wku.ac.kr.
Abstract
OBJECTIVE: To examinie the synergistic effects of Banxia Xiexin Decoction (, Known as Banhasasim-tang in Korean) extract (BXDE) on cisplatin-induced cytotoxicity in the A549 human lung cancer cell lines. METHODS: A549 cells were treated with varying concentrations (50-200 μg/mL) of cisplatin and BXDE alone or in combination for 96 h. We used 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan assay and flow cytometry to analyze cell viability and apoptosis, respectively. RESULTS: The exposure of cells to cisplatin and BXDE alone or in combination decreased cell viability dose- and time-dependently (P<0.05), which was found to be mediated by the apoptotic pathway as confirmed by the increase in the annexin V+/propidium iodide- stained cell population and a ladder pattern of discontinuous DNA fragments. Furthermore, the apoptosis was inhibited by the pan-caspase inhibitor, benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (z-VAD-FMK). CONCLUSIONS: BXDE significantly potentiated apoptotic effects of cisplatin in A549 cells. Moreover, apoptosis induced by BXDE might be the pivotal mechanism mediating its chemopreventative action against cancer.
OBJECTIVE: To examinie the synergistic effects of Banxia Xiexin Decoction (, Known as Banhasasim-tang in Korean) extract (BXDE) on cisplatin-induced cytotoxicity in the A549 humanlung cancer cell lines. METHODS: A549 cells were treated with varying concentrations (50-200 μg/mL) of cisplatin and BXDE alone or in combination for 96 h. We used 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan assay and flow cytometry to analyze cell viability and apoptosis, respectively. RESULTS: The exposure of cells to cisplatin and BXDE alone or in combination decreased cell viability dose- and time-dependently (P<0.05), which was found to be mediated by the apoptotic pathway as confirmed by the increase in the annexin V+/propidium iodide- stained cell population and a ladder pattern of discontinuous DNA fragments. Furthermore, the apoptosis was inhibited by the pan-caspase inhibitor, benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (z-VAD-FMK). CONCLUSIONS:BXDE significantly potentiated apoptotic effects of cisplatin in A549 cells. Moreover, apoptosis induced by BXDE might be the pivotal mechanism mediating its chemopreventative action against cancer.