Neil S Lagali1, Stephan Allgeier2, Pedro Guimarães3, Reza A Badian4,5,6, Alfredo Ruggeri3, Bernd Köhler2, Tor Paaske Utheim4,5, Beatrice Peebo1, Magnus Peterson7, Lars B Dahlin8, Olov Rolandsson9. 1. Department of Ophthalmology, Institute for Clinical and Experimental Medicine, Linköping University, Linköping, Sweden. 2. Institute for Applied Computer Science, Karlsruhe Institute of Technology, Karlsruhe, Germany. 3. Department of Information Engineering, University of Padova, Padova, Italy. 4. Faculty of Health Sciences, University College of Southeast Norway, Kongsberg, Norway. 5. Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway. 6. Department of Ophthalmology, Stavanger University Hospital, Stavanger/Clinical Institute 1, Faculty of Medicine, University of Bergen, Bergen, Norway. 7. Department of Public Health and Caring Sciences, Section of Family Medicine and Preventive Medicine, Uppsala University, Uppsala, Sweden. 8. Department of Translational Medicine-Hand Surgery, Lund University, Skåne University Hospital, Malmö, Sweden. 9. Department of Public Health and Clinical Medicine, Section of Family Medicine, Umeå University, Umeå, Sweden.
Abstract
Purpose: To determine if corneal subbasal nerve plexus (SBP) parameters derived from wide-area depth-corrected mosaic images are associated with type 2 diabetes. Methods: One hundred sixty-three mosaics were produced from eyes of 82 subjects by laser-scanning in vivo confocal microscopy (IVCM). Subjects were of the same age, without (43 subjects) or with type 2 diabetes (39 subjects). Mosaic corneal nerve fiber length density (mCNFL) and apical whorl corneal nerve fiber length density (wCNFL) were quantified and related to the presence and duration of diabetes (short duration < 10 years and long duration ≥ 10 years). Results: In mosaics with a mean size of 6 mm2 in subjects aged 69.1 ± 1.2 years, mCNFL in type 2 diabetes was reduced relative to nondiabetic subjects (13.1 ± 4.2 vs. 15.0 ± 3.2 mm/mm2, P = 0.018). Also reduced relative to nondiabetic subjects was mCNFL in both short-duration (14.0 ± 4.0 mm/mm2, 3.2 ± 3.9 years since diagnosis) and long-duration diabetes (12.7 ± 4.2 mm/mm2, 15.4 ± 4.2 years since diagnosis; ANOVA P = 0.023). Lower mCNFL was associated with presence of diabetes (P = 0.032) and increased hemoglobin A1c (HbA1c) levels (P = 0.047). By contrast, wCNFL was unaffected by diabetes or HbA1c (P > 0.05). Global SBP patterns revealed marked degeneration of secondary nerve fiber branches outside the whorl region in long-duration diabetes. Conclusions: Wide-area mosaic images provide reference values for mCNFL and wCNFL and reveal a progressive degeneration of the SBP with increasing duration of type 2 diabetes.
Purpose: To determine if corneal subbasal nerve plexus (SBP) parameters derived from wide-area depth-corrected mosaic images are associated with type 2 diabetes. Methods: One hundred sixty-three mosaics were produced from eyes of 82 subjects by laser-scanning in vivo confocal microscopy (IVCM). Subjects were of the same age, without (43 subjects) or with type 2 diabetes (39 subjects). Mosaic corneal nerve fiber length density (mCNFL) and apical whorl corneal nerve fiber length density (wCNFL) were quantified and related to the presence and duration of diabetes (short duration < 10 years and long duration ≥ 10 years). Results: In mosaics with a mean size of 6 mm2 in subjects aged 69.1 ± 1.2 years, mCNFL in type 2 diabetes was reduced relative to nondiabetic subjects (13.1 ± 4.2 vs. 15.0 ± 3.2 mm/mm2, P = 0.018). Also reduced relative to nondiabetic subjects was mCNFL in both short-duration (14.0 ± 4.0 mm/mm2, 3.2 ± 3.9 years since diagnosis) and long-duration diabetes (12.7 ± 4.2 mm/mm2, 15.4 ± 4.2 years since diagnosis; ANOVA P = 0.023). Lower mCNFL was associated with presence of diabetes (P = 0.032) and increased hemoglobin A1c (HbA1c) levels (P = 0.047). By contrast, wCNFL was unaffected by diabetes or HbA1c (P > 0.05). Global SBP patterns revealed marked degeneration of secondary nerve fiber branches outside the whorl region in long-duration diabetes. Conclusions: Wide-area mosaic images provide reference values for mCNFL and wCNFL and reveal a progressive degeneration of the SBP with increasing duration of type 2 diabetes.
Authors: Aleksandra Matuszewska-Iwanicka; Bernd Stratmann; Oliver Stachs; Stephan Allgeier; Andreas Bartschat; Karsten Winter; Rudolf Guthoff; Diethelm Tschoepe; Hans-Joachim Hettlich Journal: Ophthalmol Ther Date: 2022-10-03
Authors: Neil S Lagali; Stephan Allgeier; Pedro Guimarães; Reza A Badian; Alfredo Ruggeri; Bernd Köhler; Tor Paaske Utheim; Beatrice Peebo; Magnus Peterson; Lars B Dahlin; Olov Rolandsson Journal: Sci Data Date: 2018-04-24 Impact factor: 6.444
Authors: Neil S Lagali; Reza A Badian; Xu Liu; Tobias R Feldreich; Johan Ärnlöv; Tor Paaske Utheim; Lars B Dahlin; Olov Rolandsson Journal: Sci Rep Date: 2018-09-24 Impact factor: 4.379