Rudolf A Werner1,2,3, Hiroshi Wakabayashi1,4, Xinyu Chen1,3, Mitsuru Hirano1,3, Tetsuya Shinaji1,3, Constantin Lapa1, Steven P Rowe2, Mehrbod S Javadi2, Takahiro Higuchi5,3,6. 1. Department of Nuclear Medicine, University Hospital Würzburg, Würzburg, Germany. 2. Division of Nuclear Medicine and Molecular Imaging, Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, Maryland. 3. Comprehensive Heart Failure Center, University Hospital Würzburg, Würzburg, Germany. 4. Department of Nuclear Medicine, Kanazawa University, Kanazawa, Japan; and. 5. Department of Nuclear Medicine, University Hospital Würzburg, Würzburg, Germany thiguchi@me.com. 6. Department of Bio-Medical Imaging, National Cerebral and Cardiovascular Research Center, Suita, Osaka, Japan.
Abstract
Precise regional quantitative assessment of renal function is limited with conventional 99mTc-labeled renal radiotracers. A recent study reported that the PET radiotracer 2-deoxy-2-18F-fluorosorbitol (18F-FDS) has ideal pharmacokinetics for functional renal imaging. Furthermore, 18F-FDS is available via simple reduction from routinely used 18F-FDG. We aimed to further investigate the potential of 18F-FDS PET as a functional renal imaging agent using rat models of kidney disease. Methods: Two different rat models of renal impairment were investigated: induction of acute renal failure by intramuscular administration of glycerol in the hind legs, and induction of unilateral ureteral obstruction by ligation of the left ureter. At 24 h after these procedures, dynamic 30-min 18F-FDS PET data were acquired using a dedicated small-animal PET system. Urine 18F-FDS radioactivity 30 min after radiotracer injection was measured together with coinjected 99mTc-diethylenetriaminepentaacetic acid urine activity. Results: Dynamic PET imaging demonstrated rapid 18F-FDS accumulation in the renal cortex and rapid radiotracer excretion via the kidneys in healthy control rats. On the other hand, significantly delayed renal radiotracer uptake (continuous slow uptake) was observed in acute renal failure rats and unilateral ureteral obstruction kidneys. Measured urine radiotracer concentrations of 18F-FDS and 99mTc-diethylenetriaminepentaacetic acid correlated well with each other (R = 0.84, P < 0.05). Conclusion: 18F-FDS PET demonstrated favorable kinetics for functional renal imaging in rat models of kidney diseases. 18F-FDS PET imaging, with its advantages of high spatiotemporal resolution and simple tracer production, could potentially complement or replace conventional renal scintigraphy in select cases and significantly improve the diagnostic performance of renal functional imaging.
Precise regional quantitative assessment of renal function is limited with conventional 99mTc-labeled renal radiotracers. A recent study reported that the PET radiotracer 2-deoxy-2-18F-fluorosorbitol (18F-FDS) has ideal pharmacokinetics for functional renal imaging. Furthermore, 18F-FDS is available via simple reduction from routinely used 18F-FDG. We aimed to further investigate the potential of 18F-FDS PET as a functional renal imaging agent using rat models of kidney disease. Methods: Two different rat models of renal impairment were investigated: induction of acute renal failure by intramuscular administration of glycerol in the hind legs, and induction of unilateral ureteral obstruction by ligation of the left ureter. At 24 h after these procedures, dynamic 30-min 18F-FDS PET data were acquired using a dedicated small-animal PET system. Urine 18F-FDS radioactivity 30 min after radiotracer injection was measured together with coinjected 99mTc-diethylenetriaminepentaacetic acid urine activity. Results: Dynamic PET imaging demonstrated rapid 18F-FDS accumulation in the renal cortex and rapid radiotracer excretion via the kidneys in healthy control rats. On the other hand, significantly delayed renal radiotracer uptake (continuous slow uptake) was observed in acute renal failurerats and unilateral ureteral obstruction kidneys. Measured urine radiotracer concentrations of 18F-FDS and 99mTc-diethylenetriaminepentaacetic acid correlated well with each other (R = 0.84, P < 0.05). Conclusion: 18F-FDS PET demonstrated favorable kinetics for functional renal imaging in rat models of kidney diseases. 18F-FDS PET imaging, with its advantages of high spatiotemporal resolution and simple tracer production, could potentially complement or replace conventional renal scintigraphy in select cases and significantly improve the diagnostic performance of renal functional imaging.
Authors: Rudolf A Werner; Alvaro A Ordonez; Julian Sanchez-Bautista; Charles Marcus; Constantin Lapa; Steven P Rowe; Martin G Pomper; Jeffrey P Leal; Martin A Lodge; Mehrbod S Javadi; Sanjay K Jain; Takahiro Higuchi Journal: Clin Nucl Med Date: 2019-05 Impact factor: 7.794
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Authors: Rudolf A Werner; Alexander Weich; Malte Kircher; Lilja B Solnes; Mehrbod S Javadi; Takahiro Higuchi; Andreas K Buck; Martin G Pomper; Steven P Rowe; Constantin Lapa Journal: Theranostics Date: 2018-11-29 Impact factor: 11.556
Authors: Rudolf A Werner; Xinyu Chen; Constantin Lapa; Kazuhiro Koshino; Steven P Rowe; Martin G Pomper; Mehrbod S Javadi; Takahiro Higuchi Journal: Eur J Nucl Med Mol Imaging Date: 2019-05-30 Impact factor: 9.236
Authors: Camilo A Ruiz-Bedoya; Alvaro A Ordonez; Rudolf A Werner; Donika Plyku; Mariah H Klunk; Jeff Leal; Wojciech G Lesniak; Daniel P Holt; Robert F Dannals; Takahiro Higuchi; Steven P Rowe; Sanjay K Jain Journal: J Nucl Med Date: 2020-03-20 Impact factor: 11.082
Authors: Rudolf A Werner; Xinyu Chen; Steven P Rowe; Constantin Lapa; Mehrbod S Javadi; Takahiro Higuchi Journal: Int J Cardiovasc Imaging Date: 2018-10-17 Impact factor: 2.357