Literature DB >> 2923881

Intermediary structures during membrane fusion as observed by cryo-electron microscopy.

P M Frederik1, M C Stuart, A J Verkleij.   

Abstract

Lipidic phases, containing 'lipidic particles' (dioleoylphosphatidylethanolamine/cholesterol/dioleoylphosphatidylcho lin e and cardiolipin/dimyristoylphosphatidylcholine in the presence of Ca2+) have been investigated by preparing thin films from a suspension of sonicated vesicles. These thin films were vitrified and observed 'directly' by cryo-electron microscopy in their hydrated form. The thin films show various fusion products and fusion intermediates such as lipidic particles.

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Year:  1989        PMID： 2923881     DOI： 10.1016/0005-2736(89)90445-8

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  7 in total

1.  Intermediates in membrane fusion and bilayer/nonbilayer phase transitions imaged by time-resolved cryo-transmission electron microscopy.

Authors:  D P Siegel; J L Burns; M H Chestnut; Y Talmon
Journal:  Biophys J       Date:  1989-07       Impact factor: 4.033

2.  The mechanism of lamellar-to-inverted hexagonal phase transitions in phosphatidylethanolamine: implications for membrane fusion mechanisms.

Authors:  D P Siegel; R M Epand
Journal:  Biophys J       Date:  1997-12       Impact factor: 4.033

3.  Membrane fusion and the lamellar-to-inverted-hexagonal phase transition in cardiolipin vesicle systems induced by divalent cations.

Authors:  A Ortiz; J A Killian; A J Verkleij; J Wilschut
Journal:  Biophys J       Date:  1999-10       Impact factor: 4.033

4.  The mechanism of lamellar-to-inverted hexagonal phase transitions: a study using temperature-jump cryo-electron microscopy.

Authors:  D P Siegel; W J Green; Y Talmon
Journal:  Biophys J       Date:  1994-02       Impact factor: 4.033

5.  Membrane fusion in vesicles of oligomerizable lipids.

Authors:  B J Ravoo; W D Weringa; J B Engberts
Journal:  Biophys J       Date:  1999-01       Impact factor: 4.033

Review 6.  Understanding the invisible hands of sample preparation for cryo-EM.

Authors:  Giulia Weissenberger; Rene J M Henderikx; Peter J Peters
Journal:  Nat Methods       Date:  2021-05-07       Impact factor: 47.990

7.  Cryo-EM structures from sub-nl volumes using pin-printing and jet vitrification.

Authors:  Raimond B G Ravelli; Frank J T Nijpels; Rene J M Henderikx; Giulia Weissenberger; Sanne Thewessem; Abril Gijsbers; Bart W A M M Beulen; Carmen López-Iglesias; Peter J Peters
Journal:  Nat Commun       Date:  2020-05-22       Impact factor: 14.919
  7 in total

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