| Literature DB >> 29224142 |
Fumihito Miura1,2,3, Takashi Ito4,5.
Abstract
Post-bisulfite adaptor tagging (PBAT) is a highly efficient procedure to construct libraries for whole-genome bisulfite sequencing (WGBS). PBAT attaches adaptors to bisulfite-converted genomic DNA to circumvent bisulfite-induced degradation of library DNA inherent to conventional WGBS protocols. Consequently, it enables PCR-free WGBS from nanogram quantities of mammalian DNA, thereby serving as an invaluable tool for methylomics.Entities:
Keywords: Methylome; Next-generation sequencing; PCR-free; Single-nucleotide resolution
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Year: 2018 PMID： 29224142 DOI： 10.1007/978-1-4939-7481-8_7
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745