Yuanwei Luo1, Min Liang2, Wenxia Yao3, Jifang Liu3, Qiuling Niu2, Jitao Chen3, Zhaoyu Liu3, Ming Li3, Boyun Shi2, Jinhui Pan3, Lin Zhou2, Xinke Zhou4. 1. Department of General Surgery, The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510700, People's Republic of China. 2. Department of Oncology, The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510700, People's Republic of China. 3. Centre Laboratory, The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510700, People's Republic of China. 4. Department of Oncology, The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510700, People's Republic of China. Electronic address: zxkstar@gzhmu.edu.cn.
Abstract
AIMS: Evidence shows that aberrant expression of long non-coding RNA (lncRNA) is closely associated with tumor development and progression. However, the role of lncRNA in environmental carcinogen induced gastric tumorigenesis remains largely unknown. This study aimed at investigating the function role of lncRNA in N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) induce malignantly transformed human gastric epithelial cells. MAIN METHODS: In this study, high-throughput sequencing and qRT-PCR assay revealed marked downregulation of lncRNA LOC101927497 in the malignant transformed gastric epithelial cells induced by MNNG (GES-1-T cells), gain-of-function and loss-of-function assays showed that LOC101927497 can suppress the proliferation and migration of GES-1-T cells in vitro. RNA antisense purification experiment showed that LOC101927497 interacted with miR-574-5p in GES-1-T cells the most obvious. Further studies suggested that LOC101927497 may function as a tumor suppressor by interacting with miR-574-5p. KEY FINDINGS: LncRNA LOC101927497 functions as a suppressor by interacting with miR-574-5p, thus inhibiting the malignant phenotype of GES-1-T cells. SIGNIFICANCE: To the best of our knowledge, this is the first study to demonstrate the role of lncRNA in MNNG-induced gastric tumorigenesis, and it will provide new insights into the role of lncRNA in environmental carcinogen-induced gastric cancer.
AIMS: Evidence shows that aberrant expression of long non-coding RNA (lncRNA) is closely associated with tumor development and progression. However, the role of lncRNA in environmental carcinogen induced gastric tumorigenesis remains largely unknown. This study aimed at investigating the function role of lncRNA in N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) induce malignantly transformed human gastric epithelial cells. MAIN METHODS: In this study, high-throughput sequencing and qRT-PCR assay revealed marked downregulation of lncRNA LOC101927497 in the malignant transformed gastric epithelial cells induced by MNNG (GES-1-T cells), gain-of-function and loss-of-function assays showed that LOC101927497 can suppress the proliferation and migration of GES-1-T cells in vitro. RNA antisense purification experiment showed that LOC101927497 interacted with miR-574-5p in GES-1-T cells the most obvious. Further studies suggested that LOC101927497 may function as a tumor suppressor by interacting with miR-574-5p. KEY FINDINGS: LncRNA LOC101927497 functions as a suppressor by interacting with miR-574-5p, thus inhibiting the malignant phenotype of GES-1-T cells. SIGNIFICANCE: To the best of our knowledge, this is the first study to demonstrate the role of lncRNA in MNNG-induced gastric tumorigenesis, and it will provide new insights into the role of lncRNA in environmental carcinogen-induced gastric cancer.
Authors: Diego Alberto Bárcenas-López; Juan Carlos Núñez-Enríquez; Alfredo Hidalgo-Miranda; Fredy Omar Beltrán-Anaya; Didier Ismael May-Hau; Elva Jiménez-Hernández; Vilma Carolina Bekker-Méndez; Janet Flores-Lujano; Aurora Medina-Sansón; Edna Liliana Tamez-Gómez; Víctor Hugo López-García; José Ramón Lara-Ramos; Nora Nancy Núñez-Villegas; José Gabriel Peñaloza-González; Luz Victoria Flores-Villegas; Raquel Amador-Sánchez; Rosa Martha Espinosa-Elizondo; Jorge Alfonso Martín-Trejo; Martha Margarita Velázquez-Aviña; Laura Elizabeth Merino-Pasaye; María Luisa Pérez-Saldívar; David Aldebarán Duarte-Rodríguez; José Refugio Torres-Nava; Beatriz Cortés-Herrera; Karina Anastacia Solís-Labastida; Ana Itamar González-Ávila; Jessica Denisse Santillán-Juárez; Alejandra Jimena García-Velázquez; Haydee Rosas-Vargas; Minerva Mata-Rocha; Omar Alejandro Sepúlveda-Robles; Juan Manuel Mejía-Aranguré; Silvia Jiménez-Morales Journal: Genes (Basel) Date: 2020-03-13 Impact factor: 4.096