| Literature DB >> 29218563 |
An Hsieh1, Lanyuan Lu2, Mark R Chance1, Sichun Yang3.
Abstract
Integrative structure modeling is an emerging method for structural determination of protein-protein complexes that are challenging for conventional structural techniques. Here, we provide a practical protocol for implementing our integrated iSPOT platform by integrating three different biophysical techniques: small-angle X-ray scattering (SAXS), hydroxyl radical footprinting, and computational docking simulations. Specifically, individual techniques are described from experimental and/or computational perspectives, and complementary structural information from these different techniques are integrated for accurate characterization of the structures of large protein-protein complexes.Keywords: Computational docking simulation; Hydroxyl radical footprinting; Integrative structural biology; Protein-protein interaction; SAXS; Structural mass spectrometry; iSPOT
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Year: 2017 PMID： 29218563 DOI： 10.1007/978-981-10-6038-0_14
Source DB: PubMed Journal: Adv Exp Med Biol ISSN： 0065-2598 Impact factor: 2.622