Literature DB >> 2921247

Cloning and sequence analysis of flaA, a gene encoding a Spirochaeta aurantia flagellar filament surface antigen.

B Brahamsha1, E P Greenberg.   

Abstract

Spirochaeta aurantia DNA that coded for an antigenic determinant of the flagellin associated with the filament surface of the periplasmic flagella was isolated. When expressed in Escherichia coli, the antigenic polypeptide had an apparent molecular weight of 37,000. Sequence analysis of the antigen-encoding DNA revealed the presence of an open reading frame that determined a polypeptide with a predicted molecular weight of 31,241. This polypeptide showed a region of identity with the N-amino-terminal region of the 39,000- and 37,000-dalton flagellins of the distantly related spirochetes Treponema phagedenis and Treponema pallidum, respectively (S. J. Norris, N. W. Charon, R. G. Cook, M. D. Fuentes, and R. J. Limberger, J. Bacteriol. 170:4072-4082, 1988). The region of identity in the deduced S. aurantia polypeptide was preceded by a possible signal sequence and signal peptidase cleavage site.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2921247      PMCID： PMC209799          DOI： 10.1128/jb.171.3.1692-1697.1989

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  27 in total

1.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

Review 2.  Anatomy and chemistry of spirochetes.

Authors:  S C Holt
Journal:  Microbiol Rev       Date:  1978-03

3.  Axial fibrils of anaerobic spirochetes: ultrastructure and chemical characteristics.

Authors:  R Joseph; E Canale-Parola
Journal:  Arch Mikrobiol       Date:  1972

Review 4.  Bacterial flagella.

Authors:  M Silverman; M I Simon
Journal:  Annu Rev Microbiol       Date:  1977       Impact factor: 15.500

5.  Purification, ultrastructure, and composition of axial filaments from Leptospira.

Authors:  R K Nauman; S C Holt; C D Cox
Journal:  J Bacteriol       Date:  1969-04       Impact factor: 3.490

6.  Antigenic relatedness and N-terminal sequence homology define two classes of periplasmic flagellar proteins of Treponema pallidum subsp. pallidum and Treponema phagedenis.

Authors:  S J Norris; N W Charon; R G Cook; M D Fuentes; R J Limberger
Journal:  J Bacteriol       Date:  1988-09       Impact factor: 3.490

7.  Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Authors:  F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal:  Gene       Date:  1977       Impact factor: 3.688

8.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

9.  The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.

Authors:  J Shine; L Dalgarno
Journal:  Proc Natl Acad Sci U S A       Date:  1974-04       Impact factor: 11.205

10.  Chemotaxis in Spirochaeta aurantia.

Authors:  E P Greenberg; E Canale-Parola
Journal:  J Bacteriol       Date:  1977-04       Impact factor: 3.490
View more
  22 in total

1.  Differential regulation of the multiple flagellins in spirochetes.

Authors:  Chunhao Li; Melanie Sal; Michael Marko; Nyles W Charon
Journal:  J Bacteriol       Date:  2010-03-19       Impact factor: 3.490

2.  Genetic analysis of spirochete flagellin proteins and their involvement in motility, filament assembly, and flagellar morphology.

Authors:  Chunhao Li; Charles W Wolgemuth; Michael Marko; David G Morgan; Nyles W Charon
Journal:  J Bacteriol       Date:  2008-06-13       Impact factor: 3.490

3.  N-terminal amino acid sequences and amino acid compositions of the Spirochaeta aurantia flagellar filament polypeptides.

Authors:  J Parales; E P Greenberg
Journal:  J Bacteriol       Date:  1991-02       Impact factor: 3.490

Review 4.  Bacterial glycoproteins.

Authors:  P Messner
Journal:  Glycoconj J       Date:  1997-01       Impact factor: 2.916

5.  Identification of a 35-kilodalton serovar-cross-reactive flagellar protein, FlaB, from Leptospira interrogans by N-terminal sequencing, gene cloning, and sequence analysis.

Authors:  M Lin; O Surujballi; K Nielsen; S Nadin-Davis; G Randall
Journal:  Infect Immun       Date:  1997-10       Impact factor: 3.441

6.  Isolation, characterization, and cellular insertion of the flagella from two strains of the archaebacterium Methanospirillum hungatei.

Authors:  G Southam; M L Kalmokoff; K F Jarrell; S F Koval; T J Beveridge
Journal:  J Bacteriol       Date:  1990-06       Impact factor: 3.490

7.  The Borrelia burgdorferi 37-kilodalton immunoblot band (P37) used in serodiagnosis of early lyme disease is the flaA gene product.

Authors:  R D Gilmore; R L Murphree; A M James; S A Sullivan; B J Johnson
Journal:  J Clin Microbiol       Date:  1999-03       Impact factor: 5.948

8.  Inactivation of Serpulina hyodysenteriae flaA1 and flaB1 periplasmic flagellar genes by electroporation-mediated allelic exchange.

Authors:  E L Rosey; M J Kennedy; D K Petrella; R G Ulrich; R J Yancey
Journal:  J Bacteriol       Date:  1995-10       Impact factor: 3.490

9.  Physical and genetic map of the Serpulina hyodysenteriae B78T chromosome.

Authors:  R L Zuerner; T B Stanton
Journal:  J Bacteriol       Date:  1994-02       Impact factor: 3.490

10.  The Campylobacter sigma 54 flaB flagellin promoter is subject to environmental regulation.

Authors:  R A Alm; P Guerry; T J Trust
Journal:  J Bacteriol       Date:  1993-07       Impact factor: 3.490
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.