Literature DB >> 2920801

A basic protein from bovine brain that co-precipitates with tubulin in vitro.

H Ohmori1, M Kuba, S Matsumura, A Kumon.   

Abstract

A 193 kDa protein consisting of 58 kDa subunits, which has pI values of 8.50 and 8.65, was purified from bovine brain cytosol. It formed heavy precipitates with tubulin, and the molar ratio of tubulin dimer to this protein in the precipitate was 3.2. In contrast to microtubules containing ordinary microtubule-associated proteins, these complexes remained stable against cold and 1 mM CaCl2.

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Year:  1989        PMID: 2920801     DOI: 10.1007/BF01954860

Source DB:  PubMed          Journal:  Experientia        ISSN: 0014-4754


  20 in total

1.  Duplex microtubule is a new form of tubulin assembly induced by polycations.

Authors:  M Jacobs; P M Bennett; M J Dickens
Journal:  Nature       Date:  1975-10-23       Impact factor: 49.962

2.  A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

Authors:  R G MARTIN; B N AMES
Journal:  J Biol Chem       Date:  1961-05       Impact factor: 5.157

3.  Physical and chemical properties of purified tau factor and the role of tau in microtubule assembly.

Authors:  D W Cleveland; S Y Hwo; M W Kirschner
Journal:  J Mol Biol       Date:  1977-10-25       Impact factor: 5.469

4.  Formation of double-walled microtubules and multilayered tubulin sheets by basic proteins.

Authors:  E Unger; K J Böhm; H Müller; H Grossman; H Fenske; W Vater
Journal:  Eur J Cell Biol       Date:  1988-04       Impact factor: 4.492

5.  MAP5: a novel brain microtubule-associated protein under strong developmental regulation.

Authors:  B Riederer; R Cohen; A Matus
Journal:  J Neurocytol       Date:  1986-12

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Solubility properties of neuronal tubulin: evidence for labile and stable microtubules.

Authors:  M M Black; J M Cochran; J T Kurdyla
Journal:  Brain Res       Date:  1984-03-19       Impact factor: 3.252

8.  Interaction of tubulin with chromatin proteins. H1 and core histones.

Authors:  G Mithieux; C Alquier; B Roux; B Rousset
Journal:  J Biol Chem       Date:  1984-12-25       Impact factor: 5.157

9.  Quantification of Coomassie Blue stained proteins in polyacrylamide gels based on analyses of eluted dye.

Authors:  C Fenner; R R Traut; D T Mason; J Wikman-Coffelt
Journal:  Anal Biochem       Date:  1975-02       Impact factor: 3.365

10.  MAP3: characterization of a novel microtubule-associated protein.

Authors:  G Huber; D Alaimo-Beuret; A Matus
Journal:  J Cell Biol       Date:  1985-02       Impact factor: 10.539

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  1 in total

1.  Distribution of acetylated alpha-tubulin in brain. In situ localization and biochemical characterization.

Authors:  M Morales; E Fifková
Journal:  Cell Tissue Res       Date:  1991-09       Impact factor: 5.249

  1 in total

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