Literature DB >> 1786591

Distribution of acetylated alpha-tubulin in brain. In situ localization and biochemical characterization.

M Morales1, E Fifková.   

Abstract

We studied the solubility properties of brain acetylated alpha-tubulin, as well as the localization of this tubulin in brain tissue. Endogenous unpolymerized tubulin and cytoskeletal tubulin were fractionated after brain Triton-solubilization. Using the immunoblotting technique, we found that acetylated alpha-tubulin was recovered in the cytoskeletal fraction, and that most (92%) of the acetylated microtubules of this fraction were depolymerized by cold/Ca2+ treatment. In another set of experiments, axonal and soma-dendritic preparations were found to have equivalent amounts of acetylated alpha-tubulin. By immunogold electron microscopy, we established that acetylated microtubules are widely distributed in dendrites of the central nervous system.

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Year:  1991        PMID： 1786591     DOI： 10.1007/bf00340864

Source DB:  PubMed          Journal:  Cell Tissue Res        ISSN： 0302-766X            Impact factor:   5.249


  31 in total

1.  The expression of acetylated microtubules during axonal and dendritic growth in cerebellar macroneurons which develop in vitro.

Authors:  A Ferreira; A Cáceres
Journal:  Brain Res Dev Brain Res       Date:  1989-10-01

2.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

3.  Acetylated and detyrosinated alpha-tubulins are co-localized in stable microtubules in rat meningeal fibroblasts.

Authors:  M A Cambray-Deakin; R D Burgoyne
Journal:  Cell Motil Cytoskeleton       Date:  1987

4.  Solubility properties of neuronal tubulin: evidence for labile and stable microtubules.

Authors:  M M Black; J M Cochran; J T Kurdyla
Journal:  Brain Res       Date:  1984-03-19       Impact factor: 3.252

5.  Recycling of cold-stable microtubules: evidence that cold stability is due to substoichiometric polymer blocks.

Authors:  D Job; C T Rauch; E H Fischer; R L Margolis
Journal:  Biochemistry       Date:  1982-02-02       Impact factor: 3.162

6.  Serial analysis of microtubules in cultured rat sensory axons.

Authors:  D Bray; M B Bunge
Journal:  J Neurocytol       Date:  1981-08

7.  Purification and assay of a 145-kDa protein (STOP145) with microtubule-stabilizing and motility behavior.

Authors:  R L Margolis; C T Rauch; D Job
Journal:  Proc Natl Acad Sci U S A       Date:  1986-02       Impact factor: 11.205

8.  Establishment of a stable, acetylated microtubule bundle during neuronal commitment.

Authors:  M M Falconer; U Vielkind; D L Brown
Journal:  Cell Motil Cytoskeleton       Date:  1989

9.  Posttranslational modifications of alpha-tubulin: acetylated and detyrosinated forms in axons of rat cerebellum.

Authors:  M A Cambray-Deakin; R D Burgoyne
Journal:  J Cell Biol       Date:  1987-06       Impact factor: 10.539

10.  Posttranslational modification and microtubule stability.

Authors:  E Schulze; D J Asai; J C Bulinski; M Kirschner
Journal:  J Cell Biol       Date:  1987-11       Impact factor: 10.539
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  7 in total

1.  Expression of a Novel Ciliary Protein, IIIG9, During the Differentiation and Maturation of Ependymal Cells.

Authors:  M Cifuentes; V Baeza; P M Arrabal; R Visser; J M Grondona; N Saldivia; F Martínez; F Nualart; K Salazar
Journal:  Mol Neurobiol       Date:  2017-02-13       Impact factor: 5.590

2.  Entamoeba histolytica sirtuin EhSir2a deacetylates tubulin and regulates the number of microtubular assemblies during the cell cycle.

Authors:  Somasri Dam; Anuradha Lohia
Journal:  Cell Microbiol       Date:  2010-02-09       Impact factor: 3.715

3.  The relationship of hydrophobic tubulin with membranes in neural tissue.

Authors:  D M Beltramo; M Nuñez; A D Alonso; H S Barra
Journal:  Mol Cell Biochem       Date:  1994-12-07       Impact factor: 3.396

4.  Oncomodulin identifies different hair cell types in the mammalian inner ear.

Authors:  Dwayne D Simmons; Benton Tong; Angela D Schrader; Aubrey J Hornak
Journal:  J Comp Neurol       Date:  2010-09-15       Impact factor: 3.215

5.  In vivo and in vitro effects of diisopropyl phosphorofluoridate (DFP) on the rate of hen brain tubulin polymerization.

Authors:  R P Gupta; M B Abou-Donia
Journal:  Neurochem Res       Date:  1994-04       Impact factor: 3.996

6.  Partial Immunoblotting of 2D-Gels: A Novel Method to Identify Post-Translationally Modified Proteins Exemplified for the Myelin Acetylome.

Authors:  Kathrin Kusch; Marina Uecker; Thomas Liepold; Wiebke Möbius; Christian Hoffmann; Heinz Neumann; Hauke B Werner; Olaf Jahn
Journal:  Proteomes       Date:  2017-01-12

Review 7.  Huntington's Disease-An Outlook on the Interplay of the HTT Protein, Microtubules and Actin Cytoskeletal Components.

Authors:  Aleksandra S Taran; Lilia D Shuvalova; Maria A Lagarkova; Irina B Alieva
Journal:  Cells       Date:  2020-06-22       Impact factor: 6.600
  7 in total

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