Heather A Paul1, Kelsey H Collins2,3, Marc R Bomhof3,4, Hans J Vogel1,5, Raylene A Reimer1,3. 1. Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada. 2. Biomedical Engineering, Schulich School of Engineering, University of Calgary, Calgary, AB, Canada. 3. Faculty of Kinesiology, University of Calgary, Calgary, AB, Canada. 4. Department of Kinesiology and Physical Education, Faculty of Arts and Science, University of Lethbridge, Lethbridge, AB, Canada. 5. Department of Biological Sciences, Bio-NMR Center, University of Calgary, Calgary, AB, Canada.
Abstract
SCOPE: Maternal obesity programs metabolic dysfunction in offspring, increasing their susceptibility to obesity and metabolic diseases in later life. Moreover, pregnancy and lactation are associated with many metabolic adaptations, yet it is unclear how diet-induced maternal obesity may interrupt these processes. METHODS AND RESULTS: 1 H NMR serum metabolomics analysis was performed on samples collected pre-pregnancy and in pregnant and lactating lean and high fat/sucrose (HFS) diet-induced obese Sprague-Dawley rats to identify maternal metabolic pathways associated with developmental programming of offspring obesity. Gut microbial composition was assessed using qPCR. Offspring of HFS dams had nearly 40% higher adiposity at weaning compared to offspring of lean dams. While pregnancy and lactation were associated with distinct maternal metabolic changes common to both lean and obese dams, we identified several metabolic differences, potentially implicating dysregulated one-carbon and mammary gland metabolism in the metabolic programming of obesity. Gut microbial composition was significantly altered with obesity, and both gestation and lactation were accompanied by changes in gut microbiota. CONCLUSION: Diet-induced maternal obesity and consumption of an obesogenic maternal diet results in differential metabolic and gut microbial adaptations to pregnancy and lactation; these maladaptations may be directly involved in maternal programming of offspring susceptibility to obesity.
SCOPE: Maternal obesity programs metabolic dysfunction in offspring, increasing their susceptibility to obesity and metabolic diseases in later life. Moreover, pregnancy and lactation are associated with many metabolic adaptations, yet it is unclear how diet-induced maternal obesity may interrupt these processes. METHODS AND RESULTS: 1 H NMR serum metabolomics analysis was performed on samples collected pre-pregnancy and in pregnant and lactating lean and high fat/sucrose (HFS) diet-induced obeseSprague-Dawley rats to identify maternal metabolic pathways associated with developmental programming of offspring obesity. Gut microbial composition was assessed using qPCR. Offspring of HFS dams had nearly 40% higher adiposity at weaning compared to offspring of lean dams. While pregnancy and lactation were associated with distinct maternal metabolic changes common to both lean and obese dams, we identified several metabolic differences, potentially implicating dysregulated one-carbon and mammary gland metabolism in the metabolic programming of obesity. Gut microbial composition was significantly altered with obesity, and both gestation and lactation were accompanied by changes in gut microbiota. CONCLUSION: Diet-induced maternal obesity and consumption of an obesogenic maternal diet results in differential metabolic and gut microbial adaptations to pregnancy and lactation; these maladaptations may be directly involved in maternal programming of offspring susceptibility to obesity.
Authors: Sarah Galla; Saroj Chakraborty; Xi Cheng; Ji-Youn Yeo; Blair Mell; Nathaline Chiu; Camilla F Wenceslau; Matam Vijay-Kumar; Bina Joe Journal: J Am Heart Assoc Date: 2020-01-11 Impact factor: 5.501
Authors: Kelsey H Collins; Graham Z MacDonald; David A Hart; Ruth A Seerattan; Jaqueline L Rios; Raylene A Reimer; Walter Herzog Journal: J Sport Health Sci Date: 2019-06-15 Impact factor: 7.179