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Literature DB >> 29190022

Shotgun Sphingolipid Analysis of Human Aqueous Humor.

Anna Trzeciecka¹, Ulises Arbelo², Arturo Barron², Genea Edwards², Sruthi Sampathkumar³, Carol Toris³, Sanjoy K Bhattacharya⁴.

Abstract

This protocol provides a step-by-step guide to shotgun sphingolipid analysis of aqueous humor. We describe the Bligh and Dyer crude lipid extraction method and electrospray ionization tandem mass spectrometry (ESI-MS/MS) coupled with MZmine 2.21 data processing for identification and ratiometric quantitation of sphingosine, sphingosine-1-phosphate, sphingomyelin, and ceramide.

Entities: CellLine Chemical Disease Gene Species

Keywords: Aqueous humor; Ceramide; Electrospray ionization-tandem mass spectrometry (ESI-MS/MS); MZmine 2.21; Shotgun lipidomics; Sphingolipids; Sphingomyelin; Sphingosine; Sphingosine-1-phosphate

Mesh：

Substances：

Year: 2018 PMID： 29190022 PMCID： PMC7545364 DOI： 10.1007/978-1-4939-7407-8_10

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

21 in total

Review 1. Sphingolipids in neurodegeneration.

Authors: Norman J Haughey
Journal: Neuromolecular Med Date: 2010-08-25 Impact factor: 3.843

2. Ectopic expression of ceramide synthase 2 in neurons suppresses neurodegeneration induced by ceramide synthase 1 deficiency.

Authors: Stefka D Spassieva; Xiaojie Ji; Ye Liu; Kenneth Gable; Jacek Bielawski; Teresa M Dunn; Erhard Bieberich; Lihong Zhao
Journal: Proc Natl Acad Sci U S A Date: 2016-05-09 Impact factor: 11.205

Review 3. Multi-dimensional mass spectrometry-based shotgun lipidomics and novel strategies for lipidomic analyses.

Authors: Xianlin Han; Kui Yang; Richard W Gross
Journal: Mass Spectrom Rev Date: 2011-07-13 Impact factor: 10.946

4. Defective ceramide synthases in mice cause reduced amplitudes in electroretinograms and altered sphingolipid composition in retina and cornea.

Authors: Bianca Brüggen; Christiane Kremser; Andreas Bickert; Philipp Ebel; Katharina Vom Dorp; Konrad Schultz; Peter Dörmann; Klaus Willecke; Karin Dedek
Journal: Eur J Neurosci Date: 2016-06-01 Impact factor: 3.386

5. Quantitative densitometry of proteins stained with coomassie blue using a Hewlett Packard scanjet scanner and Scanplot software.

Authors: S G Vincent; P R Cunningham; N L Stephens; A J Halayko; J T Fisher
Journal: Electrophoresis Date: 1997-01 Impact factor: 3.535

6. Rapid and selective identification of molecular species in phosphatidylcholine and sphingomyelin by conditional neutral loss scanning and MS3.

Authors: Toshiaki Houjou; Kotoko Yamatani; Hiroki Nakanishi; Masayoshi Imagawa; Takao Shimizu; Ryo Taguchi
Journal: Rapid Commun Mass Spectrom Date: 2004 Impact factor: 2.419

7. A comparison of trabecular meshwork sphingolipids and ceramides of ocular normotensive and hypertensive states of DBA/2J mice.

Authors: Yenifer Guerra; Ayman J Aljohani; Genea Edwards; Sanjoy K Bhattacharya
Journal: J Ocul Pharmacol Ther Date: 2013-12-09 Impact factor: 2.671

Shotgun Sphingolipid Analysis of Human Aqueous Humor.

Review 1. Sphingolipids in neurodegeneration.

2. Ectopic expression of ceramide synthase 2 in neurons suppresses neurodegeneration induced by ceramide synthase 1 deficiency.

Review 3. Multi-dimensional mass spectrometry-based shotgun lipidomics and novel strategies for lipidomic analyses.

4. Defective ceramide synthases in mice cause reduced amplitudes in electroretinograms and altered sphingolipid composition in retina and cornea.

5. Quantitative densitometry of proteins stained with coomassie blue using a Hewlett Packard scanjet scanner and Scanplot software.

6. Rapid and selective identification of molecular species in phosphatidylcholine and sphingomyelin by conditional neutral loss scanning and MS3.

7. A comparison of trabecular meshwork sphingolipids and ceramides of ocular normotensive and hypertensive states of DBA/2J mice.

8. Measurement of protein using bicinchoninic acid.

9. The analysis of human serum transferrins with the PhastSystem: quantitation of microheterogeneity.

Review 10. Regulating survival and development in the retina: key roles for simple sphingolipids.

Review 1. Non-alcoholic fatty liver disease: Insights from sphingolipidomics.