Literature DB >> 2918243

Rapid detection of cutaneous herpes simplex virus infection with the polymerase chain reaction.

M Cao1, X Xiao, B Egbert, T M Darragh, T S Yen.   

Abstract

A simple and specific method for detecting herpes simplex virus infection in routinely processed paraffin-embedded biopsy specimens is described. DNA is extracted from paraffin blocks, and subjected to DNA amplification with the polymerase chain reaction. After 40 rounds, an amplified band can be detected after agarose gel electrophoresis and ethidium bromide staining. This band is specific for herpes simplex virus, because tissues infected with related viruses do not give this amplified band. We have been able to detect viral DNA in small punch skin biopsies with this procedure, which can take as little as 6 h.

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Year:  1989        PMID： 2918243     DOI： 10.1111/1523-1747.ep12277232

Source DB:  PubMed          Journal:  J Invest Dermatol        ISSN： 0022-202X            Impact factor:   8.551


  25 in total

Review 1.  Molecular diagnosis of herpes simplex virus infections in the central nervous system.

Authors:  Y W Tang; P S Mitchell; M J Espy; T F Smith; D H Persing
Journal:  J Clin Microbiol       Date:  1999-07       Impact factor: 5.948

2.  Detection of Herpes simplex virus DNA by real-time PCR.

Authors:  H H Kessler; G Mühlbauer; B Rinner; E Stelzl; A Berger; H W Dörr; B Santner; E Marth; H Rabenau
Journal:  J Clin Microbiol       Date:  2000-07       Impact factor: 5.948

3.  Epidemiology and molecular analysis of herpes simplex keratitis requiring primary penetrating keratoplasty.

Authors:  B C Branco; P A Gaudio; T P Margolis
Journal:  Br J Ophthalmol       Date:  2004-10       Impact factor: 4.638

4.  Negative association of Epstein-Barr virus or herpes simplex virus-1 with tumefactive central nervous system inflammatory demyelinating disease.

Authors:  Takao Kiriyama; Hiroshi Kataoka; Takahiko Kasai; Akitaka Nonomura; Satoshi Ueno
Journal:  J Neurovirol       Date:  2010-11-11       Impact factor: 2.643

5.  Detecting herpesvirus DNA in uveitis using the polymerase chain reaction.

Authors:  S Yamamoto; D Pavan-Langston; S Kinoshita; K Nishida; Y Shimomura; Y Tano
Journal:  Br J Ophthalmol       Date:  1996-05       Impact factor: 4.638

6.  Genomic sequences homologous to the protein kinase region of the bifunctional herpes simplex virus type 2 protein ICP10.

Authors:  C C Smith; J P Wymer; J Luo; L Aurelian
Journal:  Virus Genes       Date:  1991-07       Impact factor: 2.332

7.  Detection of herpes simplex virus using the polymerase chain reaction followed by endonuclease cleavage.

Authors:  B B Rogers; S L Josephson; S K Mak
Journal:  Am J Pathol       Date:  1991-07       Impact factor: 4.307

8.  Detection of Coxsackievirus B3 RNA in myocardial tissues by the polymerase chain reaction.

Authors:  L M Weiss; L A Movahed; M E Billingham; M L Cleary
Journal:  Am J Pathol       Date:  1991-02       Impact factor: 4.307

9.  Analysis of herpesvirus genomes in Kikuchi's disease.

Authors:  Y Sumiyoshi; M Kikuchi; T Minematu; K Ohshima; M Takeshita; Y Minamishima
Journal:  Virchows Arch       Date:  1994       Impact factor: 4.064

10.  An improved technique for the diagnosis of viral retinitis from samples of aqueous humor and vitreous.

Authors:  J Garweg; T Fenner; M Böhnke; H Schmitz
Journal:  Graefes Arch Clin Exp Ophthalmol       Date:  1993-09       Impact factor: 3.117
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