Mansour Rismanchian1, Saeid Nosouhian1, Mohammad Shahabouee2, Amin Davoudi3, Farzaneh Nourbakhshian4. 1. Dental Implants Research Center, Department of Prosthodontics, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran. 2. Torabinejad Dental Research Center and Department of Periodontology, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran. 3. Dental Implants Research Center, Department of Oral and Maxillofacial Surgery, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran. 4. Department of Oral and Maxillofacial Surgery, Ahvaz University of Medical Sciences, Ahvaz, Iran.
Abstract
PURPOSE: To evaluate the antiseptic properties of five different disinfectant techniques on three different peri-implantitis (PI) associated biofilms. METHODS: 90 implant titanium disks, with the same thickness and diameter, were prepared and randomly divided into 18 groups (n = 5) based on the microbiota strains (S. aureus, S. epidermidis and C. albicans) and using the following disinfectant techniques:soft laser therapy, photodynamic therapy (PDT), 0.12% NaOCl, 0.2% chlorhexidine, 3% H2O2, and control groups. After forming a protein layer on disk surfaces, the specimens were exposed to the microbial suspensions. After decontamination according to designated techniques, 2% Trypsin protease was administered to isolate the surviving microorganisms. Muller Hinton agar culture was used for microbiota growth. After 48-hour incubation, the standard colony forming unit (CFU) was assayed and the collected data were analyzed by Kruskal-Wallis and Mann-Whitney tests at a significance level of 0.05. RESULTS: The highest amount of CFU/ml values was shown by C. albicans, which was subjected to PDT (25.12 ± 30.23). The least disinfecting efficacy on S. epidermidis was demonstrated by the laser group (all P-values ≤ 0.01). Nevertheless, all of the groups exhibited significant differences with the control groups (all P-values < 0.01). CLINICAL SIGNIFICANCE: None of the studied disinfectant techniques had the highest lethal effects on all of the tested microbiotas. Therefore, a combination of these disinfectant techniques, for instance PDT ⁺ 3% H2O2 or 0.2% chlorhexidine, is recommended.
PURPOSE: To evaluate the antiseptic properties of five different disinfectant techniques on three different peri-implantitis (PI) associated biofilms. METHODS: 90 implant titanium disks, with the same thickness and diameter, were prepared and randomly divided into 18 groups (n = 5) based on the microbiota strains (S. aureus, S. epidermidis and C. albicans) and using the following disinfectant techniques:soft laser therapy, photodynamic therapy (PDT), 0.12% NaOCl, 0.2% chlorhexidine, 3% H2O2, and control groups. After forming a protein layer on disk surfaces, the specimens were exposed to the microbial suspensions. After decontamination according to designated techniques, 2% Trypsin protease was administered to isolate the surviving microorganisms. Muller Hinton agar culture was used for microbiota growth. After 48-hour incubation, the standard colony forming unit (CFU) was assayed and the collected data were analyzed by Kruskal-Wallis and Mann-Whitney tests at a significance level of 0.05. RESULTS: The highest amount of CFU/ml values was shown by C. albicans, which was subjected to PDT (25.12 ± 30.23). The least disinfecting efficacy on S. epidermidis was demonstrated by the laser group (all P-values ≤ 0.01). Nevertheless, all of the groups exhibited significant differences with the control groups (all P-values < 0.01). CLINICAL SIGNIFICANCE: None of the studied disinfectant techniques had the highest lethal effects on all of the tested microbiotas. Therefore, a combination of these disinfectant techniques, for instance PDT ⁺ 3% H2O2 or 0.2% chlorhexidine, is recommended.