Literature DB >> 29177741

An Assay for Detecting RNA Topoisomerase Activity.

Muzammil Ahmad1, Dongyi Xu1,2, Weidong Wang3.   

Abstract

RNA topoisomerase activity has recently been detected in multiple Type IA DNA topoisomerases from all three domains of life: bacteria, archaea, and eukarya. Many, but not all, Type IA topoisomerases are found to possess activities for not only DNA, but also RNA, suggesting that they may solve topological problems for both types of nucleic acids. Here we describe a detailed assay used by our group to detect RNA topoisomerase activity for many Type IA topoisomerases. We discuss the strategy, experimental procedures, troubleshooting, and limitations for this assay.

Entities:  

Keywords:  Circular RNA; Knot; Top3β; Topoisomerase

Mesh：

Substances：

Year:  2018        PMID： 29177741      PMCID： PMC5929105          DOI： 10.1007/978-1-4939-7459-7_12

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

Review 1.  Cellular roles of DNA topoisomerases: a molecular perspective.

Authors:  James C Wang
Journal:  Nat Rev Mol Cell Biol       Date:  2002-06       Impact factor: 94.444

Review 2.  Origin and evolution of DNA topoisomerases.

Authors:  Patrick Forterre; Simonetta Gribaldo; Danièle Gadelle; Marie-Claude Serre
Journal:  Biochimie       Date:  2007-01-04       Impact factor: 4.079

3.  An RNA topoisomerase.

Authors:  H Wang; R J Di Gate; N C Seeman
Journal:  Proc Natl Acad Sci U S A       Date:  1996-09-03       Impact factor: 11.205

4.  DNA and RNA topoisomerase activities of Top3β are promoted by mediator protein Tudor domain-containing protein 3.

Authors:  Grace Ee-Lu Siaw; I-Fen Liu; Po-Yen Lin; Michael D Been; Tao-Shih Hsieh
Journal:  Proc Natl Acad Sci U S A       Date:  2016-08-31       Impact factor: 11.205

5.  Mice lacking DNA topoisomerase IIIbeta develop to maturity but show a reduced mean lifespan.

Authors:  K Y Kwan; J C Wang
Journal:  Proc Natl Acad Sci U S A       Date:  2001-05-01       Impact factor: 11.205

6.  Deletion of TOP3β, a component of FMRP-containing mRNPs, contributes to neurodevelopmental disorders.

Authors:  Georg Stoll; Olli P H Pietiläinen; Bastian Linder; Jaana Suvisaari; Cornelia Brosi; William Hennah; Virpi Leppä; Minna Torniainen; Samuli Ripatti; Sirpa Ala-Mello; Oliver Plöttner; Karola Rehnström; Annamari Tuulio-Henriksson; Teppo Varilo; Jonna Tallila; Kati Kristiansson; Matti Isohanni; Jaakko Kaprio; Johan G Eriksson; Olli T Raitakari; Terho Lehtimäki; Marjo-Riitta Jarvelin; Veikko Salomaa; Matthew Hurles; Hreinn Stefansson; Leena Peltonen; Patrick F Sullivan; Tiina Paunio; Jouko Lönnqvist; Mark J Daly; Utz Fischer; Nelson B Freimer; Aarno Palotie
Journal:  Nat Neurosci       Date:  2013-08-04       Impact factor: 24.884

7.  Development of autoimmunity in mice lacking DNA topoisomerase 3beta.

Authors:  Kelvin Y Kwan; Rebecca J Greenwald; Subhasis Mohanty; Arlene H Sharpe; Albert C Shaw; James C Wang
Journal:  Proc Natl Acad Sci U S A       Date:  2007-05-21       Impact factor: 11.205

Review 8.  Roles of eukaryotic topoisomerases in transcription, replication and genomic stability.

Authors:  Yves Pommier; Yilun Sun; Shar-Yin N Huang; John L Nitiss
Journal:  Nat Rev Mol Cell Biol       Date:  2016-09-21       Impact factor: 113.915

9.  Top3β is an RNA topoisomerase that works with fragile X syndrome protein to promote synapse formation.

Authors:  Dongyi Xu; Weiping Shen; Rong Guo; Yutong Xue; Wei Peng; Jian Sima; Jay Yang; Alexei Sharov; Subramanya Srikantan; Jiandong Yang; David Fox; Yong Qian; Jennifer L Martindale; Yulan Piao; James Machamer; Samit R Joshi; Subhasis Mohanty; Albert C Shaw; Thomas E Lloyd; Grant W Brown; Minoru S H Ko; Myriam Gorospe; Sige Zou; Weidong Wang
Journal:  Nat Neurosci       Date:  2013-08-04       Impact factor: 24.884

10.  RNA topoisomerase is prevalent in all domains of life and associates with polyribosomes in animals.

Authors:  Muzammil Ahmad; Yutong Xue; Seung Kyu Lee; Jennifer L Martindale; Weiping Shen; Wen Li; Sige Zou; Maria Ciaramella; Hélène Debat; Marc Nadal; Fenfei Leng; Hongliang Zhang; Quan Wang; Grace Ee-Lu Siaw; Hengyao Niu; Yves Pommier; Myriam Gorospe; Tao-Shih Hsieh; Yuk-Ching Tse-Dinh; Dongyi Xu; Weidong Wang
Journal:  Nucleic Acids Res       Date:  2016-06-01       Impact factor: 16.971
  10 in total
  2 in total

Review 1.  The many lives of type IA topoisomerases.

Authors:  Anna H Bizard; Ian D Hickson
Journal:  J Biol Chem       Date:  2020-04-10       Impact factor: 5.157

2.  Topoisomerase Assays.

Authors:  John L Nitiss; Kostantin Kiianitsa; Yilun Sun; Karin C Nitiss; Nancy Maizels
Journal:  Curr Protoc       Date:  2021-10
  2 in total

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