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Literature DB >> 29155737

Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor.

Abstract

We detail the synthesis and purification of a mitochondrial calcium uptake inhibitor, [(OH2)(NH3)4Ru(µ-O)Ru(NH3)4(OH2)]5+. The optimized synthesis of this compound commences from [Ru(NH3)5Cl]Cl2 in 1 M NH4OH in a closed container, yielding a green solution. Purification is accomplished with cation-exchange chromatography. This compound is characterized and verified to be pure by UV-vis and IR spectroscopy. The mitochondrial calcium uptake inhibitory properties are assessed in permeabilized HeLa cells by fluorescence spectroscopy.

Entities: Chemical

Mesh：

Substances：

Year: 2017 PMID： 29155737 PMCID： PMC5755246 DOI： 10.3791/56527

Source DB: PubMed Journal: J Vis Exp ISSN： 1940-087X Impact factor: 1.355

12 in total

Review 1. The molecular era of the mitochondrial calcium uniporter.

Authors: Kimberli J Kamer; Vamsi K Mootha
Journal: Nat Rev Mol Cell Biol Date: 2015-08-19 Impact factor: 94.444

2. Inhibition of mitochondrial calcium ion transport by an oxo-bridged dinuclear ruthenium ammine complex.

Authors: W L Ying; J Emerson; M J Clarke; D R Sanadi
Journal: Biochemistry Date: 1991-05-21 Impact factor: 3.162

Review 3. Mitochondria: the calcium connection.

Authors: Laura Contreras; Ilaria Drago; Enrico Zampese; Tullio Pozzan
Journal: Biochim Biophys Acta Date: 2010-05-12

4. Oxygen-bridged dinuclear ruthenium amine complex specifically inhibits Ca2+ uptake into mitochondria in vitro and in situ in single cardiac myocytes.

Authors: M A Matlib; Z Zhou; S Knight; S Ahmed; K M Choi; J Krause-Bauer; R Phillips; R Altschuld; Y Katsube; N Sperelakis; D M Bers
Journal: J Biol Chem Date: 1998-04-24 Impact factor: 5.157

5. Integrative genomics identifies MCU as an essential component of the mitochondrial calcium uniporter.

Authors: Joshua M Baughman; Fabiana Perocchi; Hany S Girgis; Molly Plovanich; Casey A Belcher-Timme; Yasemin Sancak; X Robert Bao; Laura Strittmatter; Olga Goldberger; Roman L Bogorad; Victor Koteliansky; Vamsi K Mootha
Journal: Nature Date: 2011-06-19 Impact factor: 49.962

6. Synthetic Methods for the Preparation of a Functional Analogue of Ru360, a Potent Inhibitor of Mitochondrial Calcium Uptake.

Authors: Sarah R Nathan; Nicholas W Pino; Daniela M Arduino; Fabiana Perocchi; Samantha N MacMillan; Justin J Wilson
Journal: Inorg Chem Date: 2017-02-28 Impact factor: 5.165

7. A forty-kilodalton protein of the inner membrane is the mitochondrial calcium uniporter.

Authors: Diego De Stefani; Anna Raffaello; Enrico Teardo; Ildikò Szabò; Rosario Rizzuto
Journal: Nature Date: 2011-06-19 Impact factor: 49.962

8. Bcl-2 potentiates the maximal calcium uptake capacity of neural cell mitochondria.

Authors: A N Murphy; D E Bredesen; G Cortopassi; E Wang; G Fiskum
Journal: Proc Natl Acad Sci U S A Date: 1996-09-03 Impact factor: 11.205

Review 9. Mitochondrial calcium homeostasis as potential target for mitochondrial medicine.

Authors: Carlotta Giorgi; Chiara Agnoletto; Angela Bononi; Massimo Bonora; Elena De Marchi; Saverio Marchi; Sonia Missiroli; Simone Patergnani; Federica Poletti; Alessandro Rimessi; Jan M Suski; Mariusz R Wieckowski; Paolo Pinton
Journal: Mitochondrion Date: 2011-07-21 Impact factor: 4.160

10. Architecture of the mitochondrial calcium uniporter.

Authors: Kirill Oxenoid; Ying Dong; Chan Cao; Tanxing Cui; Yasemin Sancak; Andrew L Markhard; Zenon Grabarek; Liangliang Kong; Zhijun Liu; Bo Ouyang; Yao Cong; Vamsi K Mootha; James J Chou
Journal: Nature Date: 2016-05-02 Impact factor: 49.962

2 in total

1. A Selective and Cell-Permeable Mitochondrial Calcium Uniporter (MCU) Inhibitor Preserves Mitochondrial Bioenergetics after Hypoxia/Reoxygenation Injury.

Authors: Joshua J Woods; Neeharika Nemani; Santhanam Shanmughapriya; Akshay Kumar; MengQi Zhang; Sarah R Nathan; Manfred Thomas; Edmund Carvalho; Karthik Ramachandran; Subramanya Srikantan; Peter B Stathopulos; Justin J Wilson; Muniswamy Madesh
Journal: ACS Cent Sci Date: 2019-01-04 Impact factor: 14.553

2. Ruthenium(II)-Tris-pyrazolylmethane Complexes Inhibit Cancer Cell Growth by Disrupting Mitochondrial Calcium Homeostasis.

Authors: Jakub Cervinka; Alberto Gobbo; Lorenzo Biancalana; Lenka Markova; Vojtech Novohradsky; Massimo Guelfi; Stefano Zacchini; Jana Kasparkova; Viktor Brabec; Fabio Marchetti
Journal: J Med Chem Date: 2022-08-01 Impact factor: 8.039

2 in total