| Literature DB >> 2913161 |
M Hasui1, Y Hirabayashi, Y Kobayashi.
Abstract
Bacterial ingestion and hydrogen peroxide production by polymorphonuclear leukocytes (PMN) were simultaneously measured using flow cytometry and 2',7'-dichlorofluorescein diacetate and fluorescent Staphylococcus aureus. Only 100 microliters of a whole blood specimen were required for these determinations, and the results were found to be independent of the absolute numbers of PMN, making the purification and adjustment of PMN numbers unnecessary. A positive correlation between phagocytosis and hydrogen peroxide production by individual PMN was demonstrated in healthy adults.Entities:
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Year: 1989 PMID： 2913161 DOI： 10.1016/0022-1759(89)90118-x
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303