| Literature DB >> 29131159 |
Lingjuan He1,2, Yan Li1,2, Yi Li1,2, Wenjuan Pu1,2, Xiuzhen Huang1,2, Xueying Tian1,2, Yue Wang1,2, Hui Zhang1,2, Qiaozhen Liu1,2, Libo Zhang1,2, Huan Zhao1,2, Juan Tang1,2, Hongbin Ji1,3, Dongqing Cai4, Zhibo Han5, Zhongchao Han5, Yu Nie6, Shengshou Hu6, Qing-Dong Wang7, Ruilin Sun8, Jian Fei8, Fengchao Wang9, Ting Chen9, Yan Yan10, Hefeng Huang11, William T Pu12, Bin Zhou1,2,3,4,13.
Abstract
The Cre-loxP recombination system is the most widely used technology for in vivo tracing of stem or progenitor cell lineages. The precision of this genetic system largely depends on the specificity of Cre recombinase expression in targeted stem or progenitor cells. However, Cre expression in nontargeted cell types can complicate the interpretation of lineage-tracing studies and has caused controversy in many previous studies. Here we describe a new genetic lineage tracing system that incorporates the Dre-rox recombination system to enhance the precision of conventional Cre-loxP-mediated lineage tracing. The Dre-rox system permits rigorous control of Cre-loxP recombination in lineage tracing, effectively circumventing potential uncertainty of the cell-type specificity of Cre expression. Using this new system we investigated two topics of recent debates-the contribution of c-Kit+ cardiac stem cells to cardiomyocytes in the heart and the contribution of Sox9+ hepatic progenitor cells to hepatocytes in the liver. By overcoming the technical hurdle of nonspecific Cre-loxP-mediated recombination, this new technology provides more precise analysis of cell lineage and fate decisions and facilitates the in vivo study of stem and progenitor cell plasticity in disease and regeneration.Entities:
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Year: 2017 PMID： 29131159 DOI： 10.1038/nm.4437
Source DB: PubMed Journal: Nat Med ISSN： 1078-8956 Impact factor: 53.440