Ryo Ashida1,2, Yukiyasu Okamura3, Keiichi Ohshima4, Yuko Kakuda5, Katsuhiko Uesaka1, Teiichi Sugiura1, Takaaki Ito1, Yusuke Yamamoto1, Takashi Sugino5, Kenichi Urakami6, Masatoshi Kusuhara7, Ken Yamaguchi8. 1. Division of Hepato-Biliary-Pancreatic Surgery, Shizuoka Cancer Center, Shizuoka, Japan. 2. Department of Surgery, Keio University School of Medicine, Tokyo, Japan. 3. Division of Hepato-Biliary-Pancreatic Surgery, Shizuoka Cancer Center, Shizuoka, Japan yu.okamura@scchr.jp. 4. Medical Genetics Division, Shizuoka Cancer Center Research Institute, Shizuoka, Japan. 5. Division of Pathology, Shizuoka Cancer Center, Shizuoka, Japan. 6. Cancer Diagnostics Research Division, Shizuoka Cancer Center Research Institute, Shizuoka, Japan. 7. Regional Resources Division, Shizuoka Cancer Center Research Institute, Shizuoka, Japan. 8. Shizuoka Cancer Center Hospital and Research Institute, Shizuoka, Japan.
Abstract
BACKGROUND/AIM: Project HOPE (High-tech Omics-based Patient Evaluation) began in 2014 using integrated gene expression profiling (GEP) of cancer tissues as well as diathesis of each patient who underwent operation at our Institution. The aim of this study was to identify novel genes displaying altered gene expression related to the survival and early recurrence after hepatectomy for hepatocellular carcinoma (HCC) using the results of integrated GEP analysis. MATERIALS AND METHODS: The present study included 92 patients. Genes with aberrant expression were selected by the difference of expression levels with ≥10-fold change between tumor and non-tumor tissues. RESULTS: GEP analysis showed that down-regulation was frequently observed in the PRSS8 (64%), CYP3A4 (61%) and EPCAM (57%) genes. Multivariate analysis revealed tumor stage ≥II (p=0.008) and down-regulation of the CYP3A4 gene (p=0.036) as independent predictor for overall survival. Furthermore, multivariate analysis identified maximum tumor diameter ≥74mm (p=0.008), presence of intrahepatic-metastasis (p=0.020), and down-regulation of CYP3A4 gene (p=0.019) as independent predictors for early recurrence. CONCLUSION: CYP3A4 was identified as a novel tumor suppressor gene related to a poor prognosis in HCC. Copyright
BACKGROUND/AIM: Project HOPE (High-tech Omics-based Patient Evaluation) began in 2014 using integrated gene expression profiling (GEP) of cancer tissues as well as diathesis of each patient who underwent operation at our Institution. The aim of this study was to identify novel genes displaying altered gene expression related to the survival and early recurrence after hepatectomy for hepatocellular carcinoma (HCC) using the results of integrated GEP analysis. MATERIALS AND METHODS: The present study included 92 patients. Genes with aberrant expression were selected by the difference of expression levels with ≥10-fold change between tumor and non-tumor tissues. RESULTS: GEP analysis showed that down-regulation was frequently observed in the PRSS8 (64%), CYP3A4 (61%) and EPCAM (57%) genes. Multivariate analysis revealed tumor stage ≥II (p=0.008) and down-regulation of the CYP3A4 gene (p=0.036) as independent predictor for overall survival. Furthermore, multivariate analysis identified maximum tumor diameter ≥74mm (p=0.008), presence of intrahepatic-metastasis (p=0.020), and down-regulation of CYP3A4 gene (p=0.019) as independent predictors for early recurrence. CONCLUSION:CYP3A4 was identified as a novel tumor suppressor gene related to a poor prognosis in HCC. Copyright
Authors: A Brazma; P Hingamp; J Quackenbush; G Sherlock; P Spellman; C Stoeckert; J Aach; W Ansorge; C A Ball; H C Causton; T Gaasterland; P Glenisson; F C Holstege; I F Kim; V Markowitz; J C Matese; H Parkinson; A Robinson; U Sarkans; S Schulze-Kremer; J Stewart; R Taylor; J Vilo; M Vingron Journal: Nat Genet Date: 2001-12 Impact factor: 38.330
Authors: Eun Jung Chung; Young Kwan Sung; Mohammad Farooq; Younghee Kim; Sanguk Im; Won Young Tak; Yoon Jin Hwang; Yang Il Kim; Hyung Soo Han; Jung-Chul Kim; Moon Kyu Kim Journal: Mol Cells Date: 2002-12-31 Impact factor: 5.034