Literature DB >> 2910842

Novel O-linked carbohydrate chains in the cellulase complex (cellulosome) of Clostridium thermocellum. 3-O-Methyl-N-acetylglucosamine as a constituent of a glycoprotein.

G J Gerwig1, P de Waard, J P Kamerling, J F Vliegenthart, E Morgenstern, R Lamed, E A Bayer.   

Abstract

Alkaline borohydride treatment of the cellulosome of Clostridium thermocellum yielded two major oligosaccharide-alditols, namely D-Galp-beta(1----4)-D-GalOH and (formula; see text) The compounds, isolated via gel permeation chromatography and high performance liquid chromatography, were analyzed by monosaccharide analysis, methylation analysis, gas-liquid chromatography/mass spectrometry, fast atom bombardment/mass spectrometry, and one- and two-dimensional 500-MHz (COSY, HOHAHA, ROESY) 1H NMR spectroscopy. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis combined with blotting technology indicated that the tetrasaccharide is mainly associated with one of the cellulosome subunits.

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Year:  1989        PMID： 2910842

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  24 in total

1.  An intron-containing glycoside hydrolase family 9 cellulase gene encodes the dominant 90 kDa component of the cellulosome of the anaerobic fungus Piromyces sp. strain E2.

Authors:  Peter J M Steenbakkers; Wimal Ubhayasekera; Harry J A M Goossen; Erik M H M van Lierop; Chris van der Drift; Godfried D Vogels; Sherry L Mowbray; Huub J M Op den Camp
Journal:  Biochem J       Date:  2002-07-01       Impact factor: 3.857

Review 2.  The cellulose paradox: pollutant par excellence and/or a reclaimable natural resource?

Authors:  E A Bayer; R Lamed
Journal:  Biodegradation       Date:  1992       Impact factor: 3.909

3.  Draft genome sequences for Clostridium thermocellum wild-type strain YS and derived cellulose adhesion-defective mutant strain AD2.

Authors:  Steven D Brown; Raphael Lamed; Ely Morag; Ilya Borovok; Yuval Shoham; Dawn M Klingeman; Courtney M Johnson; Zamin Yang; Miriam L Land; Sagar M Utturkar; Martin Keller; Edward A Bayer
Journal:  J Bacteriol       Date:  2012-06       Impact factor: 3.490

Review 4.  Cellulase, clostridia, and ethanol.

Authors:  Arnold L Demain; Michael Newcomb; J H David Wu
Journal:  Microbiol Mol Biol Rev       Date:  2005-03       Impact factor: 11.056

5.  Anomalous dissociative behavior of the major glycosylated component of the cellulosome of Clostridium thermocellum.

Authors:  E Morag; E A Bayer; R Lamed
Journal:  Appl Biochem Biotechnol       Date:  1991-08       Impact factor: 2.926

6.  Biochemical Characterization of a Protease Involved in the Processing of a Streptomyces reticuli Cellulase (Avicelase).

Authors:  M Moormann; A Schlochtermeier; H Schrempf
Journal:  Appl Environ Microbiol       Date:  1993-05       Impact factor: 4.792

Review 7.  Bacterial glycoproteins.

Authors:  P Messner
Journal:  Glycoconj J       Date:  1997-01       Impact factor: 2.916

8.  Streptomyces lividans glycosylates the linker region of a beta-1,4-glycanase from Cellulomonas fimi.

Authors:  E Ong; D G Kilburn; R C Miller; R A Warren
Journal:  J Bacteriol       Date:  1994-02       Impact factor: 3.490

9.  The cellulosome system of Acetivibrio cellulolyticus includes a novel type of adaptor protein and a cell surface anchoring protein.

Authors:  Qi Xu; Wenchen Gao; Shi-You Ding; Rina Kenig; Yuval Shoham; Edward A Bayer; Raphael Lamed
Journal:  J Bacteriol       Date:  2003-08       Impact factor: 3.490

Review 10.  Evolution and pathophysiology of the human natural anti-alpha-galactosyl IgG (anti-Gal) antibody.

Authors:  U Galili
Journal:  Springer Semin Immunopathol       Date:  1993
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