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Literature DB >> 291035

Production and characterization of a monoclonal antibody to chicken type I collagen.

T F Linsenmayer, M J Hendrix, C D Little.

Abstract

We have shown that lymphocyte-myeloma cell hybridization can be used to produce large amounts of extremely high-titer specific antibodies against type I collagen, a macromolecule normally of low immunogenicity. In a passive hemagglutination assay the antibody had a high titer against chicken type I collagen but showed no activity against chicken type II or rat type I collagen. By using a two-step fluorescence histochemical procedure on sections of embryonic chicken tibia, strong fluorescence was observed in the perichondrium and surrounding connective tissue (known to contain type I collagen) but not over the cartilage (characterized by type II collagen). When used in conjunction with Staphylococcus aureus as a solid phase immunoadsorbant, the antibody was shown to bind to labeled collagen synthesized in vitro by embryonic chicken calvaria.

Entities: Disease Gene Species

Mesh：

Substances：
Collagen

Year: 1979 PMID： 291035 PMCID： PMC383901 DOI： 10.1073/pnas.76.8.3703

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

23 in total

1. SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS.

Authors: J W LITTLEFIELD
Journal: Science Date: 1964-08-14 Impact factor: 47.728

2. Differentiation in erythroleukemic cells and their somatic hybrids.

Authors: S H Orkin; F I Harosi; P Leder
Journal: Proc Natl Acad Sci U S A Date: 1975-01 Impact factor: 11.205

3. Embryonic neural retina collagen: in vitro synthesis of high molecular weight forms of type II plus a new genetic type.

Authors: T F Linsenmayer; C D Little
Journal: Proc Natl Acad Sci U S A Date: 1978-07 Impact factor: 11.205

Review 4. Newer knowledge of skeletogenesis: macromolecular transitions in the extracellular matrix.

Authors: B P Toole; T F Linsenmayer
Journal: Clin Orthop Relat Res Date: 1977 Nov-Dec Impact factor: 4.176

5. Assignment of a type I collagen structural gene to human chromosome 7.

Authors: B Sykes; E Solomon
Journal: Nature Date: 1978-04-06 Impact factor: 49.962

Review 6. Origin and distribution of collagens in the developing avian cornea.

Authors: E D Hay; T F Linsenmayer; R L Trelstad; K von der Mark
Journal: Curr Top Eye Res Date: 1979

7. Genetics of the connective tissue proteins: assignment of the gene for human type I procollagen to chromosome 17 by analysis of cell hybrids and microcell hybrids.

Authors: C V Sundar Raj; R L Church; L A Klobutcher; F H Ruddle
Journal: Proc Natl Acad Sci U S A Date: 1977-10 Impact factor: 11.205

8. A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells.

Authors: M L Gefter; D H Margulies; M D Scharff
Journal: Somatic Cell Genet Date: 1977-03

9. Simultaneous synthesis of types I and III collagen by fibroblasts in culture.

Authors: S Gay; G R Martin; P K Muller; R Timpl; K Kuhn
Journal: Proc Natl Acad Sci U S A Date: 1976-11 Impact factor: 11.205

10. Synthesis of two collagen types by embryonic chick corneal epithelium in vitro.

Authors: T F Linsenmayer; G N Smith; E D Hay
Journal: Proc Natl Acad Sci U S A Date: 1977-01 Impact factor: 11.205

19 in total

1. Extracellular matrix modifications in the interdigital spaces of the chick embryo leg bud during the formation of ectopic digits.

Authors: J M Hurle; A Colombatti
Journal: Anat Embryol (Berl) Date: 1996-04

2. Characterization of monoclonal antibody specific for human type II collagen: possible implication in collagen-induced arthritis.

Authors: T Hirofuji; K Kakimoto; H Hori; Y Nagai; K Saisho; A Sumiyoshi; T Koga
Journal: Clin Exp Immunol Date: 1985-10 Impact factor: 4.330

3. Assembly of a novel cartilage matrix protein filamentous network: molecular basis of differential requirement of von Willebrand factor A domains.

Authors: Q Chen; Y Zhang; D M Johnson; P F Goetinck
Journal: Mol Biol Cell Date: 1999-07 Impact factor: 4.138

Production and characterization of a monoclonal antibody to chicken type I collagen.

1. SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS.

2. Differentiation in erythroleukemic cells and their somatic hybrids.

3. Embryonic neural retina collagen: in vitro synthesis of high molecular weight forms of type II plus a new genetic type.

Review 4. Newer knowledge of skeletogenesis: macromolecular transitions in the extracellular matrix.

5. Assignment of a type I collagen structural gene to human chromosome 7.

Review 6. Origin and distribution of collagens in the developing avian cornea.

7. Genetics of the connective tissue proteins: assignment of the gene for human type I procollagen to chromosome 17 by analysis of cell hybrids and microcell hybrids.

8. A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells.

9. Simultaneous synthesis of types I and III collagen by fibroblasts in culture.

10. Synthesis of two collagen types by embryonic chick corneal epithelium in vitro.

1. Extracellular matrix modifications in the interdigital spaces of the chick embryo leg bud during the formation of ectopic digits.

2. Characterization of monoclonal antibody specific for human type II collagen: possible implication in collagen-induced arthritis.

3. Assembly of a novel cartilage matrix protein filamentous network: molecular basis of differential requirement of von Willebrand factor A domains.

4. [Effect of synthetic hydroxyapatite ceramics on long-term cultures of isolated chondrocytes].

Review 5. Hybridomas: a new dimension in biological analyses.

6. Production and characterization of a monoclonal antibody to human Type IV collagen.

7. Organization of collagen types I and V in the embryonic chicken cornea: monoclonal antibody studies.

8. Immunohistological and ultrastructural study of the developing tendons of the avian foot.

9. The distribution of mesenchyme proteoglycan (PG-M) during wing bud outgrowth.

10. Monoclonal antibody to human basement membrane collagen type IV.