Patrick M Meyer Sauteur1,2,3, Ruben C A de Groot1, Silvia C Estevão1, Theo Hoogenboezem1, Adrianus C J M de Bruijn1, Marcel Sluijter1, Marjolein J W de Bruijn4, Ismé M De Kleer4, Rien van Haperen5, Judith M A van den Brand6, Debby Bogaert7, Pieter L A Fraaij2,6, Cornelis Vink1,8, Rudi W Hendriks4, Janneke N Samsom9, Wendy W J Unger1, Annemarie M C van Rossum2. 1. Laboratory of Pediatrics, Erasmus MC University Medical Center-Sophia Children's Hospital, Rotterdam, The Netherlands. 2. Department of Pediatrics, Division of Pediatric Infectious Diseases and Immunology, Erasmus MC University Medical Center-Sophia Children's Hospital, Rotterdam, The Netherlands. 3. Division of Infectious Diseases and Hospital Epidemiology, Children's Research Center, University Children's Hospital Zurich, Switzerland. 4. Department of Pulmonary Medicine, University Medical Center, Rotterdam, The Netherlands. 5. Department of Cell Biology and Genetics, University Medical Center, Rotterdam, The Netherlands. 6. Department of Viroscience, Erasmus MC, University Medical Center, Rotterdam, The Netherlands. 7. Department of Pediatric Immunology and Infectious Diseases, Wilhelmina Children Hospital, University Medical Center, Utrecht, The Netherlands. 8. Erasmus University College, Erasmus University, Rotterdam, The Netherlands. 9. Laboratory of Pediatrics, Division of Gastroenterology and Nutrition, Erasmus MC University Medical Center-Sophia Children's Hospital, Rotterdam, The Netherlands.
Abstract
Background: Carriage of Mycoplasma pneumoniae (Mp) in the nasopharynx is considered a prerequisite for pulmonary infection. It is interesting to note that Mp carriage is also detected after infection. Although B cells are known to be involved in pulmonary Mp clearance, their role in Mp carriage is unknown. Methods: In this study, we show in a mouse model that Mp persists in the nose after pulmonary infection, similar to humans. Results: Infection of mice enhanced Mp-specific immunoglobulin (Ig) M and IgG levels in serum and bronchoalveolar lavage fluid. However, nasal washes only contained elevated Mp-specific IgA. These differences in Ig compartmentalization correlated with differences in Mp-specific B cell responses between nose- and lung-draining lymphoid tissues. Moreover, transferred Mp-specific serum Igs had no effect on nasal carriage in B cell-deficient μMT mice, whereas this enabled μMT mice to clear pulmonary Mp infection. Conclusions: We report the first evidence that humoral immunity is limited in clearing Mp from the upper respiratory tract.
Background: Carriage of Mycoplasma pneumoniae (Mp) in the nasopharynx is considered a prerequisite for pulmonary infection. It is interesting to note that Mp carriage is also detected after infection. Although B cells are known to be involved in pulmonary Mp clearance, their role in Mp carriage is unknown. Methods: In this study, we show in a mouse model that Mp persists in the nose after pulmonary infection, similar to humans. Results: Infection of mice enhanced Mp-specific immunoglobulin (Ig) M and IgG levels in serum and bronchoalveolar lavage fluid. However, nasal washes only contained elevated Mp-specific IgA. These differences in Ig compartmentalization correlated with differences in Mp-specific B cell responses between nose- and lung-draining lymphoid tissues. Moreover, transferred Mp-specific serum Igs had no effect on nasal carriage in B cell-deficient μMT mice, whereas this enabled μMT mice to clear pulmonary Mp infection. Conclusions: We report the first evidence that humoral immunity is limited in clearing Mp from the upper respiratory tract.
Authors: Patrick M Meyer Sauteur; Adrianus C J M de Bruijn; Catarina Graça; Anne P Tio-Gillen; Silvia C Estevão; Theo Hoogenboezem; Rudi W Hendriks; Christoph Berger; Bart C Jacobs; Annemarie M C van Rossum; Ruth Huizinga; Wendy W J Unger Journal: Infect Immun Date: 2019-01-24 Impact factor: 3.441
Authors: L Patrick Schenck; Joshua J C McGrath; Daphnée Lamarche; Martin R Stämpfli; Dawn M E Bowdish; Michael G Surette Journal: mSphere Date: 2020-12-16 Impact factor: 4.389