Literature DB >> 290851

Isolation of a recombination deficient Agrobacterium tumefaciens mutant.

P M Klapwijk, P van Beelen, R A Schilperoort.   

Abstract

The isolation of a recombination deficient (Rec-) strain of Agrobacterium tumefaciens is described. Strain LBA 4011 was mutagenized with nitrosoguanidine and after segregation 18,000 colonies were replica plated and UV irradiated. Twentytwo UV sensitive strains were isolated and tested for methylmethanesulphonate (MMS) sensitivity. Six of these strains were more MMS-sensitive than LBA 4011. A Ti plasmid that was genetically marked with Tn 1 (CbR) was introduced in these strains and the rescue of the CbR marker during superinfection with an incompatible cointegrate plasmid Ti::R 702 was determined. One strain exhibited a large reduction in rescue frequency. It is concluded that the latter strain was recombination deficient. This property did not influence the induction of plant tumours.

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Year:  1979        PMID： 290851     DOI： 10.1007/bf00330307

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  16 in total

1.  An enrichment technique for auxotrophs of Agrobacterium tumefaciens using a combination of carbenicillin and lysozyme.

Authors:  P M Klapwijk; A J de Jonge; R A Schilperoort; A Rörsch
Journal:  J Gen Microbiol       Date:  1975-11

2.  Plasmid required for virulence of Agrobacterium tumefaciens.

Authors:  B Watson; T C Currier; M P Gordon; M D Chilton; E W Nester
Journal:  J Bacteriol       Date:  1975-07       Impact factor: 3.490

3.  Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis.

Authors:  M D Chilton; M H Drummond; D J Merio; D Sciaky; A L Montoya; M P Gordon; E W Nester
Journal:  Cell       Date:  1977-06       Impact factor: 41.582

4.  Translocatable elements in procaryotes.

Authors:  N Kleckner
Journal:  Cell       Date:  1977-05       Impact factor: 41.582

5.  Transposition of ampicillin resistance from RP4 to other replicons.

Authors:  R W Hedges; A E Jacob
Journal:  Mol Gen Genet       Date:  1974

6.  Host ranges of R factors.

Authors:  N Datta; R W Hedges
Journal:  J Gen Microbiol       Date:  1972-05

7.  Transfer of virulence in vivo and in vitro in Agrobacterium.

Authors:  A Kerr; P Manigault; J Tempé
Journal:  Nature       Date:  1977-02-10       Impact factor: 49.962

Review 8.  Recombination deficient mutants of E. coli and other bacteria.

Authors:  A J Clark
Journal:  Annu Rev Genet       Date:  1973       Impact factor: 16.830

9.  Octopine and nopaline metabolism in Agrobacterium tumefaciens and crown gall tumor cells: role of plasmid genes.

Authors:  A L Montoya; M D Chilton; M P Gordon; D Sciaky; E W Nester
Journal:  J Bacteriol       Date:  1977-01       Impact factor: 3.490

10.  Coordinated regulation of octopine degradation and conjugative transfer of Ti plasmids in Agrobacterium tumefaciens: evidence for a common regulatory gene and separate operons.

Authors:  P M Klapwijk; T Scheulderman; R A Schilperoort
Journal:  J Bacteriol       Date:  1978-11       Impact factor: 3.490
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  30 in total

1.  VirD2 gene product from the nopaline plasmid pTiC58 has at least two activities required for virulence.

Authors:  T R Steck; T S Lin; C I Kado
Journal:  Nucleic Acids Res       Date:  1990-12-11       Impact factor: 16.971

2.  Agrobacterium-mediated plant transformation by novel mini-T vectors in conjunction with a high-copy vir region helper plasmid.

Authors:  E Zyprian; C I Kado
Journal:  Plant Mol Biol       Date:  1990-08       Impact factor: 4.076

3.  pSa causes oncogenic suppression of Agrobacterium by inhibiting VirE2 protein export.

Authors:  L Y Lee; S B Gelvin; C I Kado
Journal:  J Bacteriol       Date:  1999-01       Impact factor: 3.490

4.  Extension of the host range of Escherichia coli vectors by incorporation of RSF1010 replication and mobilization functions.

Authors:  U B Priefer; R Simon; A Pühler
Journal:  J Bacteriol       Date:  1985-07       Impact factor: 3.490

5.  virF, the host-range-determining virulence gene of Agrobacterium tumefaciens, affects T-DNA transfer to Zea mays.

Authors:  E Jarchow; N H Grimsley; B Hohn
Journal:  Proc Natl Acad Sci U S A       Date:  1991-12-01       Impact factor: 11.205

6.  T-DNA transfer to maize cells: histochemical investigation of beta-glucuronidase activity in maize tissues.

Authors:  W H Shen; J Escudero; M Schläppi; C Ramos; B Hohn; Z Koukolíková-Nicola
Journal:  Proc Natl Acad Sci U S A       Date:  1993-02-15       Impact factor: 11.205

7.  Negative control of octopine degradation and transfer genes of octopine Ti plasmids in Agrobacterium tumefaciens.

Authors:  P M Klapwijk; R A Schilperoort
Journal:  J Bacteriol       Date:  1979-08       Impact factor: 3.490

8.  A functional map of the nopaline catabolism genes on the Ti plasmid of Agrobacterium tumefaciens C58.

Authors:  C L Schardl; C I Kado
Journal:  Mol Gen Genet       Date:  1983

9.  Complementation analysis of Agrobacterium tumefaciens Ti plasmid mutations affecting oncogenicity.

Authors:  H J Klee; M P Gordon; E W Nester
Journal:  J Bacteriol       Date:  1982-04       Impact factor: 3.490

10.  Units of genetic expression in the virulence region of a plant tumor-inducing plasmid of Agrobacterium tumefaciens.

Authors:  V N Iyer; H J Klee; E W Nester
Journal:  Mol Gen Genet       Date:  1982
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