Literature DB >> 1104767

An enrichment technique for auxotrophs of Agrobacterium tumefaciens using a combination of carbenicillin and lysozyme.

P M Klapwijk, A J de Jonge, R A Schilperoort, A Rörsch.   

Abstract

A procedure to enrich for auxotrophic and fermentation mutants of Agrobacterium tumefaciens is described. The method is based on the amplification of the killing power of carbenicillin by the addition of lysozyme. Isolation frequencies of some types of mutants are presented, with and without the application of the proposed procedure. The yield of mutants is usually enhanced a hundredfold per enrichment treatment.

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Year:  1975        PMID： 1104767     DOI： 10.1099/00221287-91-1-177

Source DB:  PubMed          Journal:  J Gen Microbiol        ISSN： 0022-1287


  12 in total

1.  Transformation of cotton (Gossypium hirsutum L.) by Agrobacterium tumefaciens and regeneration of transgenic plants.

Authors:  E Firoozabady; D L Deboer; D J Merlo; E L Halk; L N Amerson; K E Rashka; E E Murray
Journal:  Plant Mol Biol       Date:  1987-03       Impact factor: 4.076

2.  Auxotrophy in rhizobia revisited.

Authors:  Attar S Yadav
Journal:  Indian J Microbiol       Date:  2008-01-11       Impact factor: 2.461

3.  Isolation of a recombination deficient Agrobacterium tumefaciens mutant.

Authors:  P M Klapwijk; P van Beelen; R A Schilperoort
Journal:  Mol Gen Genet       Date:  1979-06-07

4.  On the isolation of TI-plasmid from Agrobacterium tumefaciens.

Authors:  A M Ledeboer; A J Krol; J J Dons; F Spier; R A Schilperoort; I Zaenen; N van Larebeke; J Schell
Journal:  Nucleic Acids Res       Date:  1976-02       Impact factor: 16.971

5.  Ti plasmid-controlled chromosome transfer in Agrobacterium tumefaciens.

Authors:  Y Dessaux; A Petit; J G Ellis; C Legrain; M Demarez; J M Wiame; M Popoff; J Tempe
Journal:  J Bacteriol       Date:  1989-11       Impact factor: 3.490

6.  Hairy roots - a short cut to transgenic root nodules.

Authors:  J Hansen; J E Jørgensen; J Stougaard; K A Marcker
Journal:  Plant Cell Rep       Date:  1989-05       Impact factor: 4.570

7.  Plasmid transfer within and between serologically distinct strains of Rhizobium japonicum, using antibiotic resistance mutants and auxotrophs.

Authors:  W P Pilacinski; E L Schmidt
Journal:  J Bacteriol       Date:  1981-02       Impact factor: 3.490

8.  Transposition of Tn904 encoding streptomycin resistance into the octopine Ti plasmid of Agrobacterium tumefaciens.

Authors:  P M Klapwijk; J van Breukelen; K Korevaar; G Ooms; R A Schilperoort
Journal:  J Bacteriol       Date:  1980-01       Impact factor: 3.490

9.  Revertible hydrogen uptake-deficient mutants of Rhizobium japonicum.

Authors:  J E Lepo; R E Hickok; M A Cantrell; S A Russell; H J Evans
Journal:  J Bacteriol       Date:  1981-05       Impact factor: 3.490

10.  Coordinated regulation of octopine degradation and conjugative transfer of Ti plasmids in Agrobacterium tumefaciens: evidence for a common regulatory gene and separate operons.

Authors:  P M Klapwijk; T Scheulderman; R A Schilperoort
Journal:  J Bacteriol       Date:  1978-11       Impact factor: 3.490
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