C Xu1, Q Huang1, C Zhang1, W Xu2, G Xu1, X Zhao3, X Liu4, Y Du5. 1. Department of Hepatobiliary and Pancreatic Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, 20 Yuhuangding Road, Yantai, Shandong, 264000, China. 2. Department of Reproductive Center, Zibo Maternal and Child Health Care Hospital, 1 Xingyuan Road, Zibo, Shangdong, 255029, China. 3. Department of Transplantation and Hepatic Surgery, PLA No. 107 Hospital, 7 Zhichu Road, Yantai, Shandong, 264000, China. 4. Department of Hepatobiliary and Pancreatic Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, 20 Yuhuangding Road, Yantai, Shandong, 264000, China. liu634@263.net. 5. Department of Transplantation and Hepatic Surgery, PLA No. 107 Hospital, 7 Zhichu Road, Yantai, Shandong, 264000, China. 13863842543@163.com.
Abstract
BACKGROUND: There is increasing evidence that long non-coding RNAs are involved in hepatocellular carcinoma (HCC) tumorigenesis. The expression level of TRPM2-AS in HCC and its clinical association remain poorly defined. METHOD: qRT-PCR was performed to detect the expression of TRPM2-AS in 108 HCC patients. The correlations between TRPM2-AS expression and clinicopathological factors and prognosis were evaluated. The inference of TRPM2-AS to the proliferation and apoptosis of HCC cells was detected. AIMS: The aim of our study was to explore the expression of TRPM2-AS in hepatocellular carcinoma (HCC) and the relation with prognosis and clinical features. RESULTS: The expression of TRPM2-AS was higher in most HCC tissues and was significantly correlated with tumor size, AJCC stage, tumor differentiation, and the prognosis of HCC patients. Interfering TRPM2-AS expression using siRNA significantly inhibited cell proliferation and promoted cell apoptosis in two HCC cell lines. CONCLUSION: Long non-coding RNA TRPM2-AS is upregulated in HCC and represents a new biomarker for HCC and the inhibition of TRPM2-AS promotes apoptosis in HCC cells in vitro.
BACKGROUND: There is increasing evidence that long non-coding RNAs are involved in hepatocellular carcinoma (HCC) tumorigenesis. The expression level of TRPM2-AS in HCC and its clinical association remain poorly defined. METHOD: qRT-PCR was performed to detect the expression of TRPM2-AS in 108 HCC patients. The correlations between TRPM2-AS expression and clinicopathological factors and prognosis were evaluated. The inference of TRPM2-AS to the proliferation and apoptosis of HCC cells was detected. AIMS: The aim of our study was to explore the expression of TRPM2-AS in hepatocellular carcinoma (HCC) and the relation with prognosis and clinical features. RESULTS: The expression of TRPM2-AS was higher in most HCC tissues and was significantly correlated with tumor size, AJCC stage, tumor differentiation, and the prognosis of HCC patients. Interfering TRPM2-AS expression using siRNA significantly inhibited cell proliferation and promoted cell apoptosis in two HCC cell lines. CONCLUSION: Long non-coding RNA TRPM2-AS is upregulated in HCC and represents a new biomarker for HCC and the inhibition of TRPM2-AS promotes apoptosis in HCC cells in vitro.
Entities:
Keywords:
Biomarker; Hepatocellular carcinoma; Long non-coding RNA; TRPM2-AS
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