R Levit1, G Savoy de Giori1,2, A de Moreno de LeBlanc1, J G LeBlanc1. 1. Centro de Referencia para Lactobacilos (CERELA-CONICET), San Miguel de Tucumán, Argentina. 2. Cátedra de Microbiología Superior, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, San Miguel de Tucumán, Argentina.
Abstract
AIM: To assess the anti-inflammatory effect associated with individual probiotic suspensions of riboflavin-producing lactic acid bacteria (LAB) in a colitis murine model. METHODS AND RESULTS: Mice intrarectally inoculated with trinitrobenzene sulfonic acid (TNBS) were orally administered with individual suspensions of riboflavin-producing strains: Lactobacillus (Lact.) plantarum CRL2130, Lact. paracasei CRL76, Lact. bulgaricus CRL871 and Streptococcus thermophilus CRL803; and a nonriboflavin-producing strain or commercial riboflavin. The extent of colonic damage and inflammation and microbial translocation to liver were evaluated. iNOs enzyme was analysed in the intestinal tissues and cytokine concentrations in the intestinal fluids. Animals given either one of the four riboflavin-producing strains showed lower macroscopic and histologic damage scores, lower microbial translocation to liver, significant decreases of iNOs+ cells in their large intestines and decreased proinflammatory cytokines, compared with mice without treatment. The administration of pure riboflavin showed similar benefits. Lact. paracasei CRL76 accompanied its anti-inflammatory effect with increased IL-10 levels demonstrating other beneficial properties in addition to the vitamin production. CONCLUSION: Administration of riboflavin-producing strains prevented the intestinal damage induced by TNBS in mice. SIGNIFICANCE AND IMPACT OF THE STUDY: Riboflavin-producing phenotype in LAB represents a potent tool to select them for preventing/treating IBD.
AIM: To assess the anti-inflammatory effect associated with individual probiotic suspensions of riboflavin-producing lactic acid bacteria (LAB) in a colitismurine model. METHODS AND RESULTS:Mice intrarectally inoculated with trinitrobenzene sulfonic acid (TNBS) were orally administered with individual suspensions of riboflavin-producing strains: Lactobacillus (Lact.) plantarum CRL2130, Lact. paracasei CRL76, Lact. bulgaricus CRL871 and Streptococcus thermophilus CRL803; and a nonriboflavin-producing strain or commercial riboflavin. The extent of colonic damage and inflammation and microbial translocation to liver were evaluated. iNOs enzyme was analysed in the intestinal tissues and cytokine concentrations in the intestinal fluids. Animals given either one of the four riboflavin-producing strains showed lower macroscopic and histologic damage scores, lower microbial translocation to liver, significant decreases of iNOs+ cells in their large intestines and decreased proinflammatory cytokines, compared with mice without treatment. The administration of pure riboflavin showed similar benefits. Lact. paracasei CRL76 accompanied its anti-inflammatory effect with increased IL-10 levels demonstrating other beneficial properties in addition to the vitamin production. CONCLUSION: Administration of riboflavin-producing strains prevented the intestinal damage induced by TNBS in mice. SIGNIFICANCE AND IMPACT OF THE STUDY: Riboflavin-producing phenotype in LAB represents a potent tool to select them for preventing/treating IBD.
Authors: Jean Guy LeBlanc; Alejandra de Moreno de LeBlanc; Romina Levit; Graciela Savoy de Giori Journal: Appl Microbiol Biotechnol Date: 2020-02-28 Impact factor: 4.813
Authors: Julius Z H von Martels; Arno R Bourgonje; Marjolein A Y Klaassen; Hassan A A Alkhalifah; Mehdi Sadaghian Sadabad; Arnau Vich Vila; Ranko Gacesa; Ruben Y Gabriëls; Robert E Steinert; Bernadien H Jansen; Marian L C Bulthuis; Hendrik M van Dullemen; Marijn C Visschedijk; Eleonora A M Festen; Rinse K Weersma; Paul de Vos; Harry van Goor; Klaas Nico Faber; Hermie J M Harmsen; Gerard Dijkstra Journal: J Crohns Colitis Date: 2020-06-19 Impact factor: 9.071