Literature DB >> 29078986

Reversed-phase chromatography with large pore superficially porous particles for high throughput immunoglobulin G2 disulfide isoform separation.

Bingchuan Wei1, Bing Zhang2, Barry Boyes3, Yonghua Taylor Zhang2.   

Abstract

Reversed-phase liquid chromatography (RPLC) has been commonly used in IgG2 disulfide isoforms analysis. Recently, the columns packed with large pore superficially porous particles (SPP) have become available commercially. This work explores the application of this SPP technology in IgG2 disulfide isoforms separation. A high throughput and improved resolution RPLC method is developed with the optimization of column selection, gradient, temperature and flow rate. Compared with the small particles RP-UHPLC columns, large pore SPP columns provide unique selectivity and several new peaks were resolved and identified to be the free thiol variants of the IgG2 disulfide isoforms. The optimized method enables the detailed characterization of cysteines related variants in a single and fast method.
Copyright © 2017 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Disulfide bond; Free thiol; Monoclonal antibody; Protein characterization; Reversed-phase liquid chromatography; Superficially porous particle

Mesh：

Substances：

Year:  2017        PMID： 29078986      PMCID： PMC9248992          DOI： 10.1016/j.chroma.2017.10.040

Source DB:  PubMed          Journal:  J Chromatogr A        ISSN： 0021-9673            Impact factor:   4.601


  32 in total

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Journal:  J Chromatogr A       Date:  2000-08-18       Impact factor: 4.759

2.  Resolving disulfide structural isoforms of IgG2 monoclonal antibodies by ion mobility mass spectrometry.

Authors:  Dhanashri Bagal; John F Valliere-Douglass; Alain Balland; Paul D Schnier
Journal:  Anal Chem       Date:  2010-08-15       Impact factor: 6.986

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Review 4.  Shell particles, trials, tribulations and triumphs.

Authors:  Georges Guiochon; Fabrice Gritti
Journal:  J Chromatogr A       Date:  2011-02-23       Impact factor: 4.759

Review 5.  Applications of superficially porous particles: high speed, high efficiency or both?

Authors:  Xiaoli Wang; William E Barber; William J Long
Journal:  J Chromatogr A       Date:  2011-07-30       Impact factor: 4.759

6.  Protected hinge in the immunoglobulin G2-A2 disulfide isoform.

Authors:  Yaoqing Diana Liu; Robert Y-T Chou; Thomas M Dillon; Leszek Poppe; Chris Spahr; Stone D H Shi; Gregory C Flynn
Journal:  Protein Sci       Date:  2014-10-23       Impact factor: 6.725

7.  Conformational difference in human IgG2 disulfide isoforms revealed by hydrogen/deuterium exchange mass spectrometry.

Authors:  Aming Zhang; Jing Fang; Robert Y-T Chou; Pavel V Bondarenko; Zhongqi Zhang
Journal:  Biochemistry       Date:  2015-03-05       Impact factor: 3.162

8.  Reaction of protein disulfide groups with Ellman's reagent: a case study of the number of sulfhydryl and disulfide groups in Aspergillus oryzae -amylase, papain, and lysozyme.

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Journal:  Arch Biochem Biophys       Date:  1971-11       Impact factor: 4.013

Review 9.  Disulfide bond structures of IgG molecules: structural variations, chemical modifications and possible impacts to stability and biological function.

Authors:  Hongcheng Liu; Kimberly May
Journal:  MAbs       Date:  2012 Jan-Feb       Impact factor: 5.857

10.  Human IgG2 antibodies display disulfide-mediated structural isoforms.

Authors:  Jette Wypych; Ming Li; Amy Guo; Zhongqi Zhang; Theresa Martinez; Martin J Allen; Szilan Fodor; Drew N Kelner; Gregory C Flynn; Yaoqing Diana Liu; Pavel V Bondarenko; Margaret Speed Ricci; Thomas M Dillon; Alain Balland
Journal:  J Biol Chem       Date:  2008-03-13       Impact factor: 5.157
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  1 in total

1.  Importance of Particle Pore Size in Determining Retention and Selectivity in Reversed Phase Liquid Chromatography.

Authors:  Justin M Godinho; Joseph A Naese; Alexander E Toler; Barry E Boyes; Richard A Henry; Joseph J DeStefano; James P Grinias
Journal:  J Chromatogr A       Date:  2020-11-10       Impact factor: 4.759
  1 in total

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