Alireza Esmailzadeh1, Hossein Zarrinfar2, AbdolMajid Fata1,3, Tanuka Sen4. 1. Department of Parasitology and Mycology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. 2. Allergy research center, Mashhad University of Medical Sciences, Mashhad, Iran. 3. Cutaneous Leishmaniasis Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. 4. School of Life and Environmental Sciences, Faculty of Science Engineering and Built Environment, Deakin University, Burwood, Australia.
Abstract
BACKGROUND: Among the fungal pathogens, Candida species are the most common cause of urinary tract infection (UTI). Some predisposing factors such as diabetes mellitus, urinary retention, urinary stasis, renal transplantation, and hospitalization can increase the risk of candiduria. The aim of this cross-sectional study was to evaluate candiduria among type 2 diabetic patients and identification of the Candida isolates. METHOD: Four hundred clean-catch midstream urine specimens were obtained from patients with type 2 diabetes mellitus. The specimens were centrifuged and the sediments were examined by direct examination and cultured on Sabouraud dextrose agar. The plates were incubated for 2-3 days at 35°C. The Candida colonies were counted and purified using CHROMagar Candida. The isolates were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) system. RESULTS: Of the 400 urine specimens, 40 (10%) had positive cultures for Candida species with a colony count of ≥1 × 103 colony forming units (CFU)/mL. The frequencies of the Candida species were as follows: C. albicans (n = 19, 47.5%), C. glabrata (n = 15, 37.5%), C. kefyer (n = 4, 10%) and C. krusei (n = 2, 5%). Seventy-three (88%) of the patients with candiduria had hemoglobin A1c (HbA1c) levels above 7%. CONCLUSION: The rate of candiduria was relatively high in type 2 diabetic patients and they were also suffering from a lack of proper blood glucose control. Although the frequency of non-albicans Candida species had not significantly higher than C. albicans, however, they obtained more from those with symptomatic candiduria.
BACKGROUND: Among the fungal pathogens, Candida species are the most common cause of urinary tract infection (UTI). Some predisposing factors such as diabetes mellitus, urinary retention, urinary stasis, renal transplantation, and hospitalization can increase the risk of candiduria. The aim of this cross-sectional study was to evaluate candiduria among type 2 diabeticpatients and identification of the Candida isolates. METHOD: Four hundred clean-catch midstream urine specimens were obtained from patients with type 2 diabetes mellitus. The specimens were centrifuged and the sediments were examined by direct examination and cultured on Sabouraud dextrose agar. The plates were incubated for 2-3 days at 35°C. The Candida colonies were counted and purified using CHROMagar Candida. The isolates were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) system. RESULTS: Of the 400 urine specimens, 40 (10%) had positive cultures for Candida species with a colony count of ≥1 × 103 colony forming units (CFU)/mL. The frequencies of the Candida species were as follows: C. albicans (n = 19, 47.5%), C. glabrata (n = 15, 37.5%), C. kefyer (n = 4, 10%) and C. krusei (n = 2, 5%). Seventy-three (88%) of the patients with candiduria had hemoglobin A1c (HbA1c) levels above 7%. CONCLUSION: The rate of candiduria was relatively high in type 2 diabeticpatients and they were also suffering from a lack of proper blood glucose control. Although the frequency of non-albicans Candida species had not significantly higher than C. albicans, however, they obtained more from those with symptomatic candiduria.
Authors: C A Binelli; M L Moretti; R S Assis; N Sauaia; P R Menezes; E Ribeiro; D C P Geiger; Y Mikami; M Miyaji; M S Oliveira; A A Barone; A S Levin Journal: Clin Microbiol Infect Date: 2006-06 Impact factor: 8.067
Authors: Lajos Forgács; Andrew M Borman; Renátó Kovács; Dávid Balázsi; Zoltán Tóth; Bence Balázs; Chiu Chun-Ju; Gábor Kardos; Ilona Kovacs; László Majoros Journal: J Fungi (Basel) Date: 2022-05-11