Jun Xie1, Ze-Bin Chen1, Song Wu1, Feng-Xia Liang1, Qing Hao1, Qian Chen1, Hua Wang2. 1. Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion, Hubei University of Chinese Medicine, Wuhan 430061, China. 2. Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion, Hubei University of Chinese Medicine, Wuhan 430061, China. hwang.tcm@china.com.
Abstract
OBJECTIVE: To observe the different effects of electroacupucnture of "Neiguan" (PC 6) grouping with other acupoints on myocardial ischemia (MI) injury, so as to provide evidence for clinical acupoint formulas. METHODS: Forty Wistar male rats were randomly divided into normal, model, EA 1[bilateral "Neiguan" (PC 6), bilateral "Zusanli" (ST 36),"Guanyuan" (CV 4)], and EA 2[bilateral "Neiguan" (PC 6), bilateral "Shenmen" (HT 7), "Danzhong" (CV 17)] groups, 10 rats in each group. The MI model was established by subcutaneous injection with ISO solution(5 mL/kg), once per day for 7 days. Both EA 1 and EA 2 groups were subjected to EA (2 Hz/100 Hz, 1 mA) for 10 min before subcutaneous injection, once daily for 21 days. Electrocardiogram (ECG) was recorded after treatment. Serum cTnT was detected by biochemistry. SOD, GSH-Px and MDA in myocardial tissues of left ventricular were measured by ELISA, and the ultrastructural changes of myocardial cells in the left ventricular were observed by electron microscope. RESULTS: Compared with the normal group, the amplitude of S-T segment and HR of ECG, cTnT and MDA contents were significantly increased in the model group (P<0.01) accompanied with seriously destroyed ultrastructure in the myocardial tissue, while SOD and GSH-Px levels were lower than those in the normal group (P<0.01). However, EA treatments reversed all the abnormal changes in the model group (P<0.05). There was no significant difference between EA 1 and EA 2 groups in the amplitude of S-T segment and HR (P>0.05); cTnT and MDA levels in the EA 1 group were lower than those in the EA 2 group (P<0.05), while SOD and GSH-Px levels were higher than those in the EA 2 group (P<0.05), accompanied with lighter degree of ultrastructure damage in the myocardial tissue in the EA 1 group. CONCLUSIONS: EA can prevent myocardial ischemic injury, and the effect of EA 1 treatment was better than that of EA 2.
OBJECTIVE: To observe the different effects of electroacupucnture of "Neiguan" (PC 6) grouping with other acupoints on myocardial ischemia (MI) injury, so as to provide evidence for clinical acupoint formulas. METHODS: Forty Wistar male rats were randomly divided into normal, model, EA 1[bilateral "Neiguan" (PC 6), bilateral "Zusanli" (ST 36),"Guanyuan" (CV 4)], and EA 2[bilateral "Neiguan" (PC 6), bilateral "Shenmen" (HT 7), "Danzhong" (CV 17)] groups, 10 rats in each group. The MI model was established by subcutaneous injection with ISO solution(5 mL/kg), once per day for 7 days. Both EA 1 and EA 2 groups were subjected to EA (2 Hz/100 Hz, 1 mA) for 10 min before subcutaneous injection, once daily for 21 days. Electrocardiogram (ECG) was recorded after treatment. Serum cTnT was detected by biochemistry. SOD, GSH-Px and MDA in myocardial tissues of left ventricular were measured by ELISA, and the ultrastructural changes of myocardial cells in the left ventricular were observed by electron microscope. RESULTS: Compared with the normal group, the amplitude of S-T segment and HR of ECG, cTnT and MDA contents were significantly increased in the model group (P<0.01) accompanied with seriously destroyed ultrastructure in the myocardial tissue, while SOD and GSH-Px levels were lower than those in the normal group (P<0.01). However, EA treatments reversed all the abnormal changes in the model group (P<0.05). There was no significant difference between EA 1 and EA 2 groups in the amplitude of S-T segment and HR (P>0.05); cTnT and MDA levels in the EA 1 group were lower than those in the EA 2 group (P<0.05), while SOD and GSH-Px levels were higher than those in the EA 2 group (P<0.05), accompanied with lighter degree of ultrastructure damage in the myocardial tissue in the EA 1 group. CONCLUSIONS: EA can prevent myocardial ischemic injury, and the effect of EA 1 treatment was better than that of EA 2.
Authors: Huan Wang; Zhen Liu; Yuanshi Liu; Zhanqi Tong; Yan Qian; Liping Chen; Bin Jiang; Mingxiong Lin; Tao Yang; Lu Gao; Mingjun Chen; Daniela Litscher; Lu Wang; Gerhard Litscher Journal: Evid Based Complement Alternat Med Date: 2018-04-02 Impact factor: 2.629