Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 A cryptophane-based "turn-on" 129Xe NMR biosensor for monitoring calmodulin.

Literature DB >> 29058007

A cryptophane-based "turn-on" ¹²⁹Xe NMR biosensor for monitoring calmodulin.

Brittany A Riggle¹, Mara L Greenberg, Yanfei Wang, Rebecca F Wissner, Serge D Zemerov, E James Petersson, Ivan J Dmochowski.

Abstract

We present the first cryptophane-based "turn-on" 129Xe NMR biosensor, employing a peptide-functionalized cryptophane to monitor the activation of calmodulin (CaM) protein in solution. In the absence of CaM binding, interaction between the peptide and cryptophane completely suppresses the hyperpolarized 129Xe-cryptophane NMR signal. Biosensor binding to Ca2+-activated CaM produces the expected 129Xe-cryptophane NMR signal.

Entities: CellLine Chemical Disease Gene Species

Mesh：

Substances：

Year: 2017 PMID： 29058007 PMCID： PMC5681859 DOI： 10.1039/c7ob02391j

Source DB: PubMed Journal: Org Biomol Chem ISSN： 1477-0520 Impact factor: 3.876

51 in total

1. Functionalized xenon as a biosensor.

Authors: M M Spence; S M Rubin; I E Dimitrov; E J Ruiz; D E Wemmer; A Pines; S Q Yao; F Tian; P G Schultz
Journal: Proc Natl Acad Sci U S A Date: 2001-09-04 Impact factor: 11.205

Review 2. Structural diversity of calmodulin binding to its target sites.

Authors: Henning Tidow; Poul Nissen
Journal: FEBS J Date: 2013-05-13 Impact factor: 5.542

3. Development of an antibody-based, modular biosensor for 129Xe NMR molecular imaging of cells at nanomolar concentrations.

Authors: Honor M Rose; Christopher Witte; Federica Rossella; Stefan Klippel; Christian Freund; Leif Schröder
Journal: Proc Natl Acad Sci U S A Date: 2014-07-28 Impact factor: 11.205

4. Cucurbit[6]uril is an ultrasensitive (129)Xe NMR contrast agent.

Authors: Yanfei Wang; Ivan J Dmochowski
Journal: Chem Commun (Camb) Date: 2015-05-28 Impact factor: 6.222

5. Comparative studies on thermostability of calmodulin, skeletal muscle troponin C and their tryptic fragments.

Authors: H Brzeska; Z Grabarek; W Drabikowski
Journal: FEBS Lett Date: 1983-03-07 Impact factor: 4.124

6. Shorter synthesis of trifunctionalized cryptophane-A derivatives.

Authors: Olena Taratula; P Aru Hill; Yubin Bai; Najat S Khan; Ivan J Dmochowski
Journal: Org Lett Date: 2011-02-18 Impact factor: 6.005

7. A water-soluble Xe@cryptophane-111 complex exhibits very high thermodynamic stability and a peculiar (129)Xe NMR chemical shift.

Authors: Robert M Fairchild; Akil I Joseph; K Travis Holman; Heather A Fogarty; Thierry Brotin; Jean-Pierre Dutasta; Céline Boutin; Gaspard Huber; Patrick Berthault
Journal: J Am Chem Soc Date: 2010-11-10 Impact factor: 15.419

A cryptophane-based "turn-on" ¹²⁹Xe NMR biosensor for monitoring calmodulin.

1. Functionalized xenon as a biosensor.

Review 2. Structural diversity of calmodulin binding to its target sites.

3. Development of an antibody-based, modular biosensor for 129Xe NMR molecular imaging of cells at nanomolar concentrations.

4. Cucurbit[6]uril is an ultrasensitive (129)Xe NMR contrast agent.

5. Comparative studies on thermostability of calmodulin, skeletal muscle troponin C and their tryptic fragments.

6. Shorter synthesis of trifunctionalized cryptophane-A derivatives.

7. A water-soluble Xe@cryptophane-111 complex exhibits very high thermodynamic stability and a peculiar (129)Xe NMR chemical shift.

8. Solution structure of calcium-free calmodulin.

Review 9. Calmodulin's flexibility allows for promiscuity in its interactions with target proteins and peptides.

10. Calcium-calmodulin modulation of the olfactory cyclic nucleotide-gated cation channel.

1. Cryptophane Nanoscale Assemblies Expand ¹²⁹Xe NMR Biosensing.

Review 2. Molecular Sensing with Host Systems for Hyperpolarized ¹²⁹Xe.

3. Monomeric Cryptophane with Record-High Xe Affinity Gives Insights into Aggregation-Dependent Sensing.

4. Cryptophane-xenon complexes for ¹²⁹Xe MRI applications.

Review 2. Structural diversity of calmodulin binding to its target sites.

Review 9. Calmodulin's flexibility allows for promiscuity in its interactions with target proteins and peptides.

Review 2. Molecular Sensing with Host Systems for Hyperpolarized 129Xe.

Review 2. Molecular Sensing with Host Systems for Hyperpolarized ¹²⁹Xe.