Farahnaz Fahimipour1, Erfan Dashtimoghadam2, Morteza Rasoulianboroujeni2, Mostafa Yazdimamaghani3, Kimia Khoshroo2, Mohammadreza Tahriri2, Amir Yadegari2, Jose A Gonzalez2, Daryoosh Vashaee4, Douglas C Lobner5, Tahereh S Jafarzadeh Kashi6, Lobat Tayebi7. 1. Marquette University School of Dentistry, Milwaukee, WI 53233, USA; Dental Biomaterials Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran. 2. Marquette University School of Dentistry, Milwaukee, WI 53233, USA. 3. School of Chemical Engineering, Oklahoma State University, Stillwater, OK 74078, USA. 4. Electrical and Computer Engineering Department, North Carolina State University, Raleigh, NC 27606, USA. 5. Department of Biomedical Sciences, Marquette University, Milwaukee, WI 53233, USA. 6. Dental Biomaterials Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: jafarzat@sina.tums.ac.ir. 7. Marquette University School of Dentistry, Milwaukee, WI 53233, USA; Department of Engineering Science, University of Oxford, Oxford OX1 3PJ, UK. Electronic address: lobat.tayebi@marquette.edu.
Abstract
OBJECTIVE: A systematic characterization of hybrid scaffolds, fabricated based on combinatorial additive manufacturing technique and freeze-drying method, is presented as a new platform for osteoblastic differentiation of dental pulp cells (DPCs). METHODS: The scaffolds were consisted of a collagenous matrix embedded in a 3D-printed beta-tricalcium phosphate (β-TCP) as the mineral phase. The developed construct design was intended to achieve mechanical robustness owing to 3D-printed β-TCP scaffold, and biologically active 3D cell culture matrix pertaining to the Collagen extracellular matrix. The β-TCP precursor formulations were investigated for their flow-ability at various temperatures, which optimized for fabrication of 3D printed scaffolds with interconnected porosity. The hybrid constructs were characterized by 3D laser scanning microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and compressive strength testing. RESULTS: The in vitro characterization of scaffolds revealed that the hybrid β-TCP/Collagen constructs offer superior DPCs proliferation and alkaline phosphatase (ALP) activity compared to the 3D-printed β-TCP scaffold over three weeks. Moreover, it was found that the incorporation of TCP into the Collagen matrix improves the ALP activity. SIGNIFICANCE: The presented results converge to suggest the developed 3D-printed β-TCP/Collagen hybrid constructs as a new platform for osteoblastic differentiation of DPCs for craniomaxillofacial bone regeneration.
OBJECTIVE: A systematic characterization of hybrid scaffolds, fabricated based on combinatorial additive manufacturing technique and freeze-drying method, is presented as a new platform for osteoblastic differentiation of dental pulp cells (DPCs). METHODS: The scaffolds were consisted of a collagenous matrix embedded in a 3D-printed beta-tricalcium phosphate (β-TCP) as the mineral phase. The developed construct design was intended to achieve mechanical robustness owing to 3D-printed β-TCP scaffold, and biologically active 3D cell culture matrix pertaining to the Collagen extracellular matrix. The β-TCP precursor formulations were investigated for their flow-ability at various temperatures, which optimized for fabrication of 3D printed scaffolds with interconnected porosity. The hybrid constructs were characterized by 3D laser scanning microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and compressive strength testing. RESULTS: The in vitro characterization of scaffolds revealed that the hybrid β-TCP/Collagen constructs offer superior DPCs proliferation and alkaline phosphatase (ALP) activity compared to the 3D-printed β-TCP scaffold over three weeks. Moreover, it was found that the incorporation of TCP into the Collagen matrix improves the ALP activity. SIGNIFICANCE: The presented results converge to suggest the developed 3D-printed β-TCP/Collagen hybrid constructs as a new platform for osteoblastic differentiation of DPCs for craniomaxillofacial bone regeneration.
Authors: Barbara Leukers; Hülya Gülkan; Stephan H Irsen; Stefan Milz; Carsten Tille; Matthias Schieker; Hermann Seitz Journal: J Mater Sci Mater Med Date: 2005-12 Impact factor: 3.896
Authors: Mehdi Razavi; Mohammadhossein Fathi; Omid Savabi; Daryoosh Vashaee; Lobat Tayebi Journal: Mater Sci Eng C Mater Biol Appl Date: 2014-11-08 Impact factor: 7.328
Authors: Sophie C Cox; John A Thornby; Gregory J Gibbons; Mark A Williams; Kajal K Mallick Journal: Mater Sci Eng C Mater Biol Appl Date: 2014-11-08 Impact factor: 7.328
Authors: Aref Shahini; Mostafa Yazdimamaghani; Kenneth J Walker; Margaret A Eastman; Hamed Hatami-Marbini; Brenda J Smith; John L Ricci; Sundar V Madihally; Daryoosh Vashaee; Lobat Tayebi Journal: Int J Nanomedicine Date: 2013-12-24
Authors: Zeynep Aytac; Nileshkumar Dubey; Arwa Daghrery; Jessica A Ferreira; Isaac J de Souza Araújo; Miguel Castilho; Jos Malda; Marco C Bottino Journal: Int Mater Rev Date: 2021-07-05 Impact factor: 15.750
Authors: N S Rodrigues; C M França; A Tahayeri; Z Ren; V P A Saboia; A J Smith; J L Ferracane; H Koo; L E Bertassoni Journal: J Dent Res Date: 2021-05-26 Impact factor: 8.924
Authors: Julia Guerrero-Gironés; Antonia Alcaina-Lorente; Clara Ortiz-Ruiz; Eduardo Ortiz-Ruiz; María P Pecci-Lloret; Antonio José Ortiz-Ruiz; Francisco Javier Rodríguez-Lozano; Miguel R Pecci-Lloret Journal: Int J Environ Res Public Health Date: 2021-04-08 Impact factor: 3.390