| Literature DB >> 29043624 |
Laura Baranello1,2, Fedor Kouzine3, Damian Wojtowicz3, Kairong Cui4, Keji Zhao4, Teresa M Przytycka5, Giovanni Capranico6, David Levens3.
Abstract
Here, we present two approaches to map DNA double-strand breaks (DSBs) and single-strand breaks (SSBs) in the genome of human cells. We named these methods respectively DSB-Seq and SSB-Seq. We tested the DSB and SSB-Seq in HCT1116, human colon cancer cells, and validated the results using the topoisomerase 2 (Top2)-poisoning agent etoposide (ETO). These methods are powerful tools for the direct detection of the physiological and pathological "breakome" of the DNA in human cells.Entities:
Keywords: DNA damage; Double-strand breaks (DSBs); Etoposide (ETO); Single-strand breaks (SSBs); Topoisomerase 2 (Top2)
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Year: 2018 PMID： 29043624 PMCID： PMC8057127 DOI： 10.1007/978-1-4939-7306-4_13
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745