Literature DB >> 29043116

A rapid and efficient DNA extraction method suitable for marine macroalgae.

Gautham Subramaniam Ramakrishnan1, Anwar Aliya Fathima1, Mohandass Ramya1.   

Abstract

Macroalgae are a diverse group of organisms. Marine macroalgae, in particular, have numerous medicinal and industrial applications. Molecular studies of macroalgae require suitable concentrations of DNA free of contaminants. At present, numerous protocols exist for DNA extraction from macroalgae. However, they are either time consuming, expensive or work only with few species. The method described in this study is rapid and efficient and applicable to different types of marine macroalgae. This method yields an average of 3.85 µg of DNA per 50 mg of algal tissue, with an average purity of 1.88. The isolated DNA was suitable for PCR amplification of universal plastid region of macroalgae.

Keywords:  Chlorophyta; DNA extraction; Genomic DNA; Macroalgae; Phaeophyceae; Rhodophyta

Year:  2017        PMID： 29043116      PMCID： PMC5628057          DOI： 10.1007/s13205-017-0992-2

Source DB:  PubMed          Journal:  3 Biotech        ISSN： 2190-5738            Impact factor:   2.406


  8 in total

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Journal:  Nature       Date:  2010-06-03       Impact factor: 49.962

4.  The cell wall polysaccharide metabolism of the brown alga Ectocarpus siliculosus. Insights into the evolution of extracellular matrix polysaccharides in Eukaryotes.

Authors:  Gurvan Michel; Thierry Tonon; Delphine Scornet; J Mark Cock; Bernard Kloareg
Journal:  New Phytol       Date:  2010-07-02       Impact factor: 10.151

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7.  A simple and effective method for high quality co-extraction of genomic DNA and total RNA from low biomass Ectocarpus siliculosus, the model brown alga.

Authors:  Maria Greco; Claudio A Sáez; Murray T Brown; Maria Beatrice Bitonti
Journal:  PLoS One       Date:  2014-05-27       Impact factor: 3.240

8.  Chemical Composition and Antioxidant and Antibacterial Activities of an Essential Oil Extracted from an Edible Seaweed, Laminaria japonica L.

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  8 in total
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3.  Biosynthesis and characterization of a recombinant eukaryotic allophycocyanin using prokaryotic accessory enzymes.

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  3 in total

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