İhsan Çetin1, İhsan Tezdiğ2, Mahmut Cem Tarakçioğlu3, Muhammed Tayyib Kadak4, Ömer Faruk Demirel5, Ömer Faruk Özer6, Fırat Erdoğan7, Burak Doğangün4. 1. Department of Nutrition and Dietetics, Batman University School of Health, Batman, Turkey. 2. Department of Chemistry, Batman University Institute of Science, Batman, Turkey. 3. Department of Child and Adolescent Psychiatry, Kanuni Sultan Süleyman Training and Research Hospital, İstanbul, Turkey. 4. Department of Child and Adolescent Psychiatry, İstanbul University Cerrahpaşa School of Medicine, İstanbul, Turkey. 5. Department of Psychiatry, İstanbul University Cerrahpaşa School of Medicine, İstanbul, Turkey. 6. Department of Biochemistry, Bezmialem Vakif University School of Medicine, İstanbul, Turkey. 7. Department of Pediatrics, Medipol University School of Medicine, İstanbul, Turkey.
Abstract
INTRODUCTION: The mechanism of ubiquitination-related abnormalities causing neural development problems is still unclear. We examined the association between autism and serum transactive response DNA-binding protein-43 (TDP-43) and ubiquitin c-terminal hydrolase-L1 (UCH-L1) levels, both of which are members of the ubiquitin-proteosome system. METHODS: We measured serum levels of TDP-43 and UCH-L1 in 24 children with autism and 24 healthy children. Childhood Autism Rating Scale (CARS) was used to assess symptom severity at admission. RESULTS: The mean serum TDP-43 and UCH-L1 levels in children with autism spectrum disorder (ASD) were found to decrease compared to healthy controls (p<0.001, 506.21±780.97 ng/L and 1245.80±996.76 ng/L, respectively; 3.08±5.44 ng/mL and 8.64±6.67 ng/mL, respectively). A positive correlation between serum TDP-43 levels and UCH-L1 levels was found in the ASD group (r=0.947, n=24, p<0.001). The CARS score of children with ASD was 48.91 points (standard deviation [SD]: 5.82). CONCLUSION: Low serum levels of UCH-L1 and TDP-43 may reflect disturbed ubiquitination in autism.
INTRODUCTION: The mechanism of ubiquitination-related abnormalities causing neural development problems is still unclear. We examined the association between autism and serum transactive response DNA-binding protein-43 (TDP-43) and ubiquitin c-terminal hydrolase-L1 (UCH-L1) levels, both of which are members of the ubiquitin-proteosome system. METHODS: We measured serum levels of TDP-43 and UCH-L1 in 24 children with autism and 24 healthy children. Childhood Autism Rating Scale (CARS) was used to assess symptom severity at admission. RESULTS: The mean serum TDP-43 and UCH-L1 levels in children with autism spectrum disorder (ASD) were found to decrease compared to healthy controls (p<0.001, 506.21±780.97 ng/L and 1245.80±996.76 ng/L, respectively; 3.08±5.44 ng/mL and 8.64±6.67 ng/mL, respectively). A positive correlation between serum TDP-43 levels and UCH-L1 levels was found in the ASD group (r=0.947, n=24, p<0.001). The CARS score of children with ASD was 48.91 points (standard deviation [SD]: 5.82). CONCLUSION: Low serum levels of UCH-L1 and TDP-43 may reflect disturbed ubiquitination in autism.
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