| Literature DB >> 29019987 |
Jian Yang1, David J Ryan1, Wei Wang1, Jason Cheuk-Ho Tsang1, Guocheng Lan2, Hideki Masaki3, Xuefei Gao1, Liliana Antunes1, Yong Yu1, Zhexin Zhu1, Juexuan Wang1, Aleksandra A Kolodziejczyk1,4, Lia S Campos1, Cui Wang1, Fengtang Yang1, Zhen Zhong5, Beiyuan Fu1, Melanie A Eckersley-Maslin6, Michael Woods1, Yosuke Tanaka7,8,9, Xi Chen1, Adam C Wilkinson7,8, James Bussell1, Jacqui White1, Ramiro Ramirez-Solis1, Wolf Reik1,6, Berthold Göttgens7,8, Sarah A Teichmann1,4, Patrick P L Tam10,11, Hiromitsu Nakauchi3,12, Xiangang Zou2, Liming Lu1,13, Pentao Liu1.
Abstract
Mouse embryonic stem cells derived from the epiblast contribute to the somatic lineages and the germline but are excluded from the extra-embryonic tissues that are derived from the trophectoderm and the primitive endoderm upon reintroduction to the blastocyst. Here we report that cultures of expanded potential stem cells can be established from individual eight-cell blastomeres, and by direct conversion of mouse embryonic stem cells and induced pluripotent stem cells. Remarkably, a single expanded potential stem cell can contribute both to the embryo proper and to the trophectoderm lineages in a chimaera assay. Bona fide trophoblast stem cell lines and extra-embryonic endoderm stem cells can be directly derived from expanded potential stem cells in vitro. Molecular analyses of the epigenome and single-cell transcriptome reveal enrichment for blastomere-specific signature and a dynamic DNA methylome in expanded potential stem cells. The generation of mouse expanded potential stem cells highlights the feasibility of establishing expanded potential stem cells for other mammalian species.Entities:
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Year: 2017 PMID： 29019987 PMCID： PMC5890884 DOI： 10.1038/nature24052
Source DB: PubMed Journal: Nature ISSN： 0028-0836 Impact factor: 49.962