Martin Fasshauer1,2, Thomas Krüwel3, Antonia Zapf4, Vera C Stahnke3, Margret Rave-Fränk5, Wieland Staab3,6, Jan M Sohns3,6, Michael Steinmetz6,7, Christina Unterberg-Buchwald3,6,8, Andreas Schuster6,8, Christian Ritter3,6, Joachim Lotz3,6. 1. Department of Diagnostic and Interventional Radiology, University Medical Center Göttingen, Göttingen Heart Center, Robert-Koch-Str. 40, 37075, Göttingen, Germany. martin.fasshauer@med.uni-goettingen.de. 2. German Centre for Cardiovascular Research (DZHK), Göttingen, Germany. martin.fasshauer@med.uni-goettingen.de. 3. Department of Diagnostic and Interventional Radiology, University Medical Center Göttingen, Göttingen Heart Center, Robert-Koch-Str. 40, 37075, Göttingen, Germany. 4. Department of Medical Statistics, University Medical Center Göttingen, Göttingen, Germany. 5. Department of Radiotherapy and Radiooncology, University Medical Center Göttingen, Göttingen, Germany. 6. German Centre for Cardiovascular Research (DZHK), Göttingen, Germany. 7. Department of Pediatric Cardiology and Intensive Care Medicine, University Medical Center Göttingen, Göttingen Heart Center, Göttingen, Germany. 8. Department of Cardiology and Pneumology, University Medical Center Göttingen, Göttingen Heart Center, Göttingen, Germany.
Abstract
OBJECTIVES: Magnetic resonance imaging (MRI) is regarded as a non-harming and non-invasive imaging modality with high tissue contrast and almost no side effects. Compared to other cross-sectional imaging modalities, MRI does not use ionising radiation. Recently, however, strong magnetic fields as applied in clinical MRI scanners have been suspected to induce DNA double-strand breaks in human lymphocytes. METHODS: In this study we investigated the impact of 3-T cardiac MRI examinations on the induction of DNA double-strand breaks in peripheral mononuclear cells by γH2AX staining and flow cytometry analysis. The study cohort consisted of 73 healthy non-smoking volunteers with 36 volunteers undergoing CMRI and 37 controls without intervention. Differences between the two cohorts were analysed by a mixed linear model with repeated measures. RESULTS: Both cohorts showed a significant increase in the γH2AX signal from baseline to post-procedure of 6.7 % (SD 7.18 %) and 7.8 % (SD 6.61 %), respectively. However, the difference between the two groups was not significant. CONCLUSION: Based on our study, γH2AX flow cytometry shows no evidence that 3-T MRI examinations as used in cardiac scans impair DNA integrity in peripheral mononuclear cells. KEY POINTS: • No evidence for DNA double-strand breaks after cardiac MRI. • Prospective study underlines safe use of MRI with regard to DNA damage. • Controlled trial involving both genders investigating DNA DSBs after 3-T MRI.
OBJECTIVES: Magnetic resonance imaging (MRI) is regarded as a non-harming and non-invasive imaging modality with high tissue contrast and almost no side effects. Compared to other cross-sectional imaging modalities, MRI does not use ionising radiation. Recently, however, strong magnetic fields as applied in clinical MRI scanners have been suspected to induce DNA double-strand breaks in human lymphocytes. METHODS: In this study we investigated the impact of 3-T cardiac MRI examinations on the induction of DNA double-strand breaks in peripheral mononuclear cells by γH2AX staining and flow cytometry analysis. The study cohort consisted of 73 healthy non-smoking volunteers with 36 volunteers undergoing CMRI and 37 controls without intervention. Differences between the two cohorts were analysed by a mixed linear model with repeated measures. RESULTS: Both cohorts showed a significant increase in the γH2AX signal from baseline to post-procedure of 6.7 % (SD 7.18 %) and 7.8 % (SD 6.61 %), respectively. However, the difference between the two groups was not significant. CONCLUSION: Based on our study, γH2AX flow cytometry shows no evidence that 3-T MRI examinations as used in cardiac scans impair DNA integrity in peripheral mononuclear cells. KEY POINTS: • No evidence for DNA double-strand breaks after cardiac MRI. • Prospective study underlines safe use of MRI with regard to DNA damage. • Controlled trial involving both genders investigating DNA DSBs after 3-T MRI.
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