| Literature DB >> 28986437 |
John A Bowden1, Alan Heckert2, Candice Z Ulmer3, Christina M Jones3, Jeremy P Koelmel4, Laila Abdullah5, Linda Ahonen6, Yazen Alnouti7, Aaron M Armando8, John M Asara9,10, Takeshi Bamba11, John R Barr12, Jonas Bergquist13, Christoph H Borchers14,15,16,17, Joost Brandsma18, Susanne B Breitkopf9, Tomas Cajka19, Amaury Cazenave-Gassiot20, Antonio Checa21, Michelle A Cinel22, Romain A Colas23, Serge Cremers24, Edward A Dennis8, James E Evans5, Alexander Fauland21, Oliver Fiehn19,25, Michael S Gardner12, Timothy J Garrett4, Katherine H Gotlinger26, Jun Han14, Yingying Huang27, Aveline Huipeng Neo20, Tuulia Hyötyläinen28, Yoshihiro Izumi11, Hongfeng Jiang24, Houli Jiang26, Jiang Jiang8, Maureen Kachman29, Reiko Kiyonami27, Kristaps Klavins30, Christian Klose31, Harald C Köfeler32, Johan Kolmert21, Therese Koal30, Grielof Koster18, Zsuzsanna Kuklenyik12, Irwin J Kurland33, Michael Leadley34, Karen Lin14, Krishna Rao Maddipati35, Danielle McDougall4, Peter J Meikle22, Natalie A Mellett22, Cian Monnin36, M Arthur Moseley37, Renu Nandakumar24, Matej Oresic38, Rainey Patterson4, David Peake27, Jason S Pierce39, Martin Post34, Anthony D Postle18, Rebecca Pugh40, Yunping Qiu33, Oswald Quehenberger41, Parsram Ramrup36, Jon Rees12, Barbara Rembiesa39, Denis Reynaud34, Mary R Roth42, Susanne Sales43, Kai Schuhmann43, Michal Laniado Schwartzman26, Charles N Serhan23, Andrej Shevchenko43, Stephen E Somerville44, Lisa St John-Williams37, Michal A Surma31, Hiroaki Takeda11, Rhishikesh Thakare7, J Will Thompson37, Federico Torta20, Alexander Triebl32, Martin Trötzmüller32, S J Kumari Ubhayasekera13, Dajana Vuckovic36, Jacquelyn M Weir22, Ruth Welti42, Markus R Wenk20, Craig E Wheelock21, Libin Yao42, Min Yuan9, Xueqing Heather Zhao33, Senlin Zhou35.
Abstract
As the lipidomics field continues to advance, self-evaluation within the community is critical. Here, we performed an interlaboratory comparison exercise for lipidomics using Standard Reference Material (SRM) 1950-Metabolites in Frozen Human Plasma, a commercially available reference material. The interlaboratory study comprised 31 diverse laboratories, with each laboratory using a different lipidomics workflow. A total of 1,527 unique lipids were measured across all laboratories and consensus location estimates and associated uncertainties were determined for 339 of these lipids measured at the sum composition level by five or more participating laboratories. These evaluated lipids detected in SRM 1950 serve as community-wide benchmarks for intra- and interlaboratory quality control and method validation. These analyses were performed using nonstandardized laboratory-independent workflows. The consensus locations were also compared with a previous examination of SRM 1950 by the LIPID MAPS consortium. While the central theme of the interlaboratory study was to provide values to help harmonize lipids, lipid mediators, and precursor measurements across the community, it was also initiated to stimulate a discussion regarding areas in need of improvement.Entities:
Keywords: National Institute of Standards and Technology; Standard Reference Material 1950; fatty acyls; glycerolipids; lipids; phospholipids; quality control; quantitation; sphingolipids; sterols
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Year: 2017 PMID： 28986437 PMCID： PMC5711491 DOI： 10.1194/jlr.M079012
Source DB: PubMed Journal: J Lipid Res ISSN： 0022-2275 Impact factor: 5.922