Literature DB >> 28980276

Quantitative Proteomic Analysis of Mass Limited Tissue Samples for Spatially Resolved Tissue Profiling.

Paul D Piehowski1, Rui Zhao2, Ronald J Moore1, Geremy Clair1, Charles Ansong3.   

Abstract

Traditionally, proteomic studies have been carried out on whole tissues or organs enabling the profiling of thousands of proteins within a single LC-MS analysis. A disadvantage of this approach is that proteomes generated from whole tissues are an "average" that represents a blend of cell types and distinct anatomical regions which can obscure important biological phenomena. Laser capture microdissection (LCM) is an elegant method that allows tissue features of interest, as small as a single cell, to be identified and isolated for downstream analysis. Herein we describe an approach that utilizes an immobilized enzyme reactor (IMER) coupled directly to nanoLC-MS/MS for highly sensitive, automated, quantitative proteomic analysis of the microscopic tissue specimens generated by LCM.

Entities:  

Keywords:  Immobilized enzyme reactor; Laser capture microdissection; Mass spectrometry; NanoLC; Nanoproteomics; Proteomics

Mesh：

Substances：

Year:  2018        PMID： 28980276      PMCID： PMC6959974          DOI： 10.1007/7651_2017_78

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  27 in total

Review 1.  Trends in ultrasensitive proteomics.

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2.  Optimized nLC-MS workflow for laser capture microdissected breast cancer tissue.

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Journal:  J Proteomics       Date:  2012-01-24       Impact factor: 4.044

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4.  Integrating accelerated tryptic digestion into proteomics workflows.

Authors:  Gordon W Slysz; David C Schriemer
Journal:  Methods Mol Biol       Date:  2009

Review 5.  Microscale immobilized enzyme reactors in proteomics: latest developments.

Authors:  Muhammad Safdar; Jens Spross; Janne Jänis
Journal:  J Chromatogr A       Date:  2013-12-01       Impact factor: 4.759

6.  Simple and Integrated Spintip-Based Technology Applied for Deep Proteome Profiling.

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Journal:  Anal Chem       Date:  2016-04-20       Impact factor: 6.986

7.  Development and evaluation of a micro- and nanoscale proteomic sample preparation method.

Authors:  Haixing Wang; Wei-Jun Qian; Heather M Mottaz; Therese R W Clauss; David J Anderson; Ronald J Moore; David G Camp; Arshad H Khan; Daniel M Sforza; Maria Pallavicini; Desmond J Smith; Richard D Smith
Journal:  J Proteome Res       Date:  2005 Nov-Dec       Impact factor: 4.466

8.  Comparative reevaluation of FASP and enhanced FASP methods by LC-MS/MS.

Authors:  Andrew J M Nel; Shaun Garnett; Jonathan M Blackburn; Nelson C Soares
Journal:  J Proteome Res       Date:  2015-02-10       Impact factor: 4.466

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Journal:  J Proteome Res       Date:  2011-05-18       Impact factor: 4.466

10.  A one-step preparation method of monolithic enzyme reactor for highly efficient sample preparation coupled to mass spectrometry-based proteomics studies.

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Journal:  J Chromatogr A       Date:  2015-08-05       Impact factor: 4.759
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2.  Determining protein polarization proteome-wide using physical dissection of individual Stentor coeruleus cells.

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Journal:  Curr Biol       Date:  2022-04-20       Impact factor: 10.900

3.  Repetitive diacetyl vapor exposure promotes ubiquitin proteasome stress and precedes bronchiolitis obliterans pathology.

Authors:  Juan Wang; So-Young Kim; Emma House; Heather M Olson; Carl J Johnston; David Chalupa; Eric Hernady; Thomas J Mariani; Gérémy Clair; Charles Ansong; Wei-Jun Qian; Jacob N Finkelstein; Matthew D McGraw
Journal:  Arch Toxicol       Date:  2021-05-24       Impact factor: 5.153

4.  Micropuncture of Bowman's Space in Mice Facilitated by 2 Photon Microscopy.

Authors:  Katsuyuki Matsushita; Kirsti Golgotiu; Daniel J Orton; Richard D Smith; Karin D Rodland; Paul D Piehowski; Michael P Hutchens
Journal:  J Vis Exp       Date:  2018-10-11       Impact factor: 1.355
  4 in total

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