THE MECHANISMS OF APOPTOSIS INITIATION IN RATS WITH CHRONIC ENTEROCOLITIS COMBINED WITH STREPTOZOTOCIN-INDUCED DIABETES.

Diabetes mellitus is a multifactorial metabolic disease, characterized by hyperglycemia due to insulin deficiency or insulin resistance. A large number of experimental studies emphasizes the key role of apoptosis in the pathogenesis of diabetes. A deeper understanding of the apoptosis mechanisms, identifying of the predictors that positively or negatively influence on the cell death initiation, the correlation between some indices will improve therapeutic strategies for patients with diabetes and comorbidities, which makes the actuality of this study. The aim of the research was to study the mechanism of cell death initiation in rats with chronic enterocolitis combined with diabetes mellitus induced by streptozotocin. The experiments were performed on 48 white nonlinear mature male rats. The animals were divided into 4 groups: the 1st - control group (n=12), the 2nd - animals with diabetes mellitus (n=12), the 3rd - animals with chronic enterocolitis (n=12), the 4th - animals with diabetes mellitus and chronic enterocolitis (n=12). Apoptotic cells of blood leukocyte suspension and reactive oxygen species were identified by flow cytometry method. Correlation analysis was performed between all the studied indices. Coefficient of linear correlation (r) and its fidelity (p) was calculated that was accordingly denoted in the tables (correlation matrices). The correlation coefficient was significant at p<0.05. Development of chronic enterocolitis combined with streptozotocin-induced diabetes in rats is accompanied by an increase in the number of leukocytes with signs of apoptosis: the percentage of V+/PI- leukocytes is greater by 24.2%, as compared to the group with diabetes (p<0.01) and by 88.0%, compared to that with chronic enterocolitis (p<0.001); The value V+/PI+ of leukocytes exceeds the results of the chronic enterocolitis-group by 50.0%. Conducted correlative analysis showed that in case of chronic enterocolitis combined with streptozotocine-induced diabetes both free radical oxidation processes and the inflammatory process take part in the development of apoptotic death of leukocytes, but a stronger association is established with reactive oxygen species.