Soheila Rezaei1,2, Mehdi Shakibaie3, Maryam Kabir-Salmani2, Mostafa Soltani Moghaddam4, Mohammad Rezvani5, Maryam Shahali6, Marzieh Naseri7. 1. Department of Agricultural Biotechnology, Payame Noor University, Tehran, Iran. 2. Department of Stem Cell and Regenerative Medicine, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran. 3. Department of Bioprocess Engineering, Institute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran. 4. Department of Stem Cell, Geneocell Science based Company, Tehran, Iran. 5. Department of Environment, Natural Resources, Payame Noor University, Tehran, Iran. 6. Department of Quality Control, Pasteur Institute of Iran, Tehran, Iran. 7. Department of Molecular Medicine, Iran University of Medical Sciences, Tehran, Iran.
Abstract
BACKGROUND: Expansion and differentiation of stem cells relies on the soluble materials as well as the physical conditions of their microenvironment. Several methods have been studied in attempt to enhance the growth and differentiation rates of different adult stem cells extracted from different sources. OBJECTIVES: The purpose was to improve the three-dimensional (3D) culture condition of the semi-permeable polymeric beads for encapsulation of the human adipose-derived mesenchymal stem cells (hADSCs) by modifying the ratio of the alginate-gelatin composition. MATERIALS AND METHODS: Following isolation and characterization of hADSCs by flow cytometry and their functional differentiation, encapsulation in the alginate and alginate/gelatin compositions were performed. Moreover, the stability, swelling, size frequency, growth kinetics, and cytotoxicity of the beads were measured to meet proper condition in the designed experimental and control culture conditions. Finally, the growth rates of the cells in different experimental groups and control were measured and analyzed statistically. RESULTS: Viability decreased in 2 and 3 percent alginate once compared to 1% alginate in beads (p≤0.05). Moreover swelling of the beads in the alginate/gelatin compositions (50:50 and 70:30) were higher than the pure alginate beads (p≤0.05). Finally, the cell growth rate in alginate/gelatin (50:50) beads was significantly higher than alginate and alginate/gelatin (70:30) beads (p≤0.05). CONCLUSIONS: These findings suggested for the first time that the composite of alginate/gelatin beads with the ratio of 50:50 might provide a suitable culture condition for the encapsulation and in vitro expansion of the hADSCs.
BACKGROUND: Expansion and differentiation of stem cells relies on the soluble materials as well as the physical conditions of their microenvironment. Several methods have been studied in attempt to enhance the growth and differentiation rates of different adult stem cells extracted from different sources. OBJECTIVES: The purpose was to improve the three-dimensional (3D) culture condition of the semi-permeable polymeric beads for encapsulation of the human adipose-derived mesenchymal stem cells (hADSCs) by modifying the ratio of the alginate-gelatin composition. MATERIALS AND METHODS: Following isolation and characterization of hADSCs by flow cytometry and their functional differentiation, encapsulation in the alginate and alginate/gelatin compositions were performed. Moreover, the stability, swelling, size frequency, growth kinetics, and cytotoxicity of the beads were measured to meet proper condition in the designed experimental and control culture conditions. Finally, the growth rates of the cells in different experimental groups and control were measured and analyzed statistically. RESULTS: Viability decreased in 2 and 3 percent alginate once compared to 1% alginate in beads (p≤0.05). Moreover swelling of the beads in the alginate/gelatin compositions (50:50 and 70:30) were higher than the pure alginate beads (p≤0.05). Finally, the cell growth rate in alginate/gelatin (50:50) beads was significantly higher than alginate and alginate/gelatin (70:30) beads (p≤0.05). CONCLUSIONS: These findings suggested for the first time that the composite of alginate/gelatin beads with the ratio of 50:50 might provide a suitable culture condition for the encapsulation and in vitro expansion of the hADSCs.
Entities:
Keywords:
3D culture; Alginate; Encapsulation; Gelatin; Stem cells
Authors: F Raquel Maia; Keila B Fonseca; Gabriela Rodrigues; Pedro L Granja; Cristina C Barrias Journal: Acta Biomater Date: 2014-03-07 Impact factor: 8.947
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