Zikang Xie1, Jianda Xu1, Libo Peng1, Yi Gao1, Hong Zhao1, Yuxing Qu2. 1. Department of orthopedics, Changzhou Traditional Chinese Medicine Hospital, Changzhou, 213003, China. 2. Department of orthopedics, Changzhou Traditional Chinese Medicine Hospital, Changzhou, 213003, China. yuxing_qu@163.com.
Abstract
OBJECTIVES: To investigate the roles of miR-149 in the progression of human osteosarcoma (OS). RESULTS: miR-149 level was upregulated in tissues from OS patients more than in normal subjects. Cell proliferation and apoptosis assays revealed that miR-149 increased cell proliferation and inhibited cell apoptosis in OS cell line (MG63). An increase of Bcl-2 gene expression and a decrease of cleaved-caspase-3, and cleaved-PARP expression were observed in MG63 cells with transfection of miR-149. Additionally, bone morphogenetic protein 9 (BMP9) was identified as a target of miR-149 in MG63 cells, and BMP9 expression was negatively correlated with miR149 level in OS clinical samples. Co-overexpression of BMP9 with miR-149 in MG63 cells prohibited miR-149-mediated promotive effects on OS progression. Importantly, overexpression of miR-149 conferred chemoresistance in MG63 cells. CONCLUSIONS: miR-149 promotes OS progression via targeting BMP9.
OBJECTIVES: To investigate the roles of miR-149 in the progression of humanosteosarcoma (OS). RESULTS:miR-149 level was upregulated in tissues from OS patients more than in normal subjects. Cell proliferation and apoptosis assays revealed that miR-149 increased cell proliferation and inhibited cell apoptosis in OS cell line (MG63). An increase of Bcl-2 gene expression and a decrease of cleaved-caspase-3, and cleaved-PARP expression were observed in MG63 cells with transfection of miR-149. Additionally, bone morphogenetic protein 9 (BMP9) was identified as a target of miR-149 in MG63 cells, and BMP9 expression was negatively correlated with miR149 level in OS clinical samples. Co-overexpression of BMP9 with miR-149 in MG63 cells prohibited miR-149-mediated promotive effects on OS progression. Importantly, overexpression of miR-149 conferred chemoresistance in MG63 cells. CONCLUSIONS:miR-149 promotes OS progression via targeting BMP9.
Entities:
Keywords:
Bcl-2; Bone morphogenetic protein 9; MG63 cells; Osteosarcoma; miR-149
Authors: G M Viera; K B Salomao; G R de Sousa; M Baroni; L E A Delsin; J A Pezuk; M S Brassesco Journal: Clin Transl Oncol Date: 2019-04-04 Impact factor: 3.405
Authors: Sami Mostafa; Mikhail Pakvasa; Elam Coalson; Allen Zhu; Alex Alverdy; Hector Castillo; Jiaming Fan; Alex Li; Yixiao Feng; Di Wu; Elliott Bishop; Scott Du; Mia Spezia; Alissa Li; Ofir Hagag; Alison Deng; Winny Liu; Mingyang Li; Sherwin S Ho; Aravind Athiviraham; Michael J Lee; Jennifer Moriatis Wolf; Guillermo A Ameer; Hue H Luu; Rex C Haydon; Jason Strelzow; Kelly Hynes; Tong-Chuan He; Russell R Reid Journal: Genes Dis Date: 2019-07-24