Literature DB >> 28945707

MECAT: fast mapping, error correction, and de novo assembly for single-molecule sequencing reads.

Chuan-Le Xiao1,2,3,4, Ying Chen2, Shang-Qian Xie1,5, Kai-Ning Chen1, Yan Wang1, Yue Han1,6, Feng Luo7, Zhi Xie1.   

Abstract

We present a tool that combines fast mapping, error correction, and de novo assembly (MECAT; accessible at https://github.com/xiaochuanle/MECAT) for processing single-molecule sequencing (SMS) reads. MECAT's computing efficiency is superior to that of current tools, while the results MECAT produces are comparable or improved. MECAT enables reference mapping or de novo assembly of large genomes using SMS reads on a single computer.

Mesh:

Year:  2017        PMID: 28945707     DOI: 10.1038/nmeth.4432

Source DB:  PubMed          Journal:  Nat Methods        ISSN: 1548-7091            Impact factor:   28.547


  16 in total

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Journal:  Nature       Date:  2016-10-05       Impact factor: 49.962

4.  Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data.

Authors:  Chen-Shan Chin; David H Alexander; Patrick Marks; Aaron A Klammer; James Drake; Cheryl Heiner; Alicia Clum; Alex Copeland; John Huddleston; Evan E Eichler; Stephen W Turner; Jonas Korlach
Journal:  Nat Methods       Date:  2013-05-05       Impact factor: 28.547

5.  Fast gapped-read alignment with Bowtie 2.

Authors:  Ben Langmead; Steven L Salzberg
Journal:  Nat Methods       Date:  2012-03-04       Impact factor: 28.547

6.  Phased diploid genome assembly with single-molecule real-time sequencing.

Authors:  Chen-Shan Chin; Paul Peluso; Fritz J Sedlazeck; Maria Nattestad; Gregory T Concepcion; Alicia Clum; Christopher Dunn; Ronan O'Malley; Rosa Figueroa-Balderas; Abraham Morales-Cruz; Grant R Cramer; Massimo Delledonne; Chongyuan Luo; Joseph R Ecker; Dario Cantu; David R Rank; Michael C Schatz
Journal:  Nat Methods       Date:  2016-10-17       Impact factor: 28.547

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8.  Hybrid error correction and de novo assembly of single-molecule sequencing reads.

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9.  Mapping single molecule sequencing reads using basic local alignment with successive refinement (BLASR): application and theory.

Authors:  Mark J Chaisson; Glenn Tesler
Journal:  BMC Bioinformatics       Date:  2012-09-19       Impact factor: 3.169

10.  Long-read sequence assembly of the gorilla genome.

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Journal:  Science       Date:  2016-04-01       Impact factor: 47.728

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Review 2.  Nanopore sequencing technology and tools for genome assembly: computational analysis of the current state, bottlenecks and future directions.

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8.  Large Differences in the Haptophyte Phaeocystis globosa Mitochondrial Genomes Driven by Repeat Amplifications.

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