Life History, Systematics and Evolution of the Diplostomoidea Poirier, 1886: Progress, Promises and Challenges Emerging From Molecular Studies.

Members of the Diplostomoidea mature in amniotes and employ vertebrates, annelids and molluscs as second intermediate hosts. Diplostomoid life cycles generally follow a three-host pattern typical of digeneans, but novelties have arisen in some species, including obligate four-host life cycles, vertical transmission, and intracellular parasitism. In this review, we summarize the basic biology of diplostomoids with reference to molecular studies, and present challenges, gaps and areas where molecular data could address long-standing questions. Our analysis of published studies revealed that most molecular surveys find more diplostomoid species than expected, but this tendency is influenced by how much effort goes into examining specimens morphologically and the number of sequenced worms. To date, molecular work has concentrated disproportionately on intraspecific or species-level diversity of larval stages in the Diplostomidae in temperate northern regions. Although the higher taxonomy of the superfamily is recognized to be in need of revision, little molecular work has been conducted at this level. Our phylogenetic analysis indicates several families and subfamilies require reconsideration, and that larval morphotypes are more reflective of evolutionary relationships than definitive hosts. The host associations of adult diplostomoids result from host-switching processes, whereas molecular surveys indicate that larval diplostomoid metacercariae have narrow ranges of second intermediate hosts, consistent with coevolution. Molecular data are often used to link diplostomoid developmental stages, and we provide data from adult Neodiplostomum and Mesoophorodiplostomum that correct earlier misidentifications of their larval stages and propose alternatives to collecting definitive hosts.